EMDataResource Pseudotorsion Plot Beta Server
RNA adopts complex three-dimensional folding patterns and has key roles in many cellular functions. Many cryoEM-derived structures contain RNA, including ribosomal particles, spliceosomes, RNA viruses, and polymerase complexes, yet procedures for validation of RNA structures are not as well developed as those for proteins.
This prototype web-service for virtual torsion angle validation of RNA structures can be applied to structures determined using cryo-EM as well as other structural methods. Virtual torsion angle plots for nucleic acids are analogous to Ramachandran plots for proteins. The tool evaluates single structures, either by file upload (mmCIF or PDB format) or by entering a PDB id.
The research community has developed simplified ways to represent nucleic acid backbone conformation through use of virtual torsion angles1-3. A common scheme is to define two virtual torsion angles (pseudotorsions), eta (η) and theta (θ) for a nucleotide triplet. The measured (η,θ) pair defines the backbone conformation relative to central residue (i).
The server calculates three varieties of pseudotorsion angles, as shown in the image below. In all definitions, phosphate atom positions are used, but the alternate atom differs: it is either the C4' atom (η,θ), C1' atom (η',θ'), or base origin4,5 position (η",θ").
Virtual torsion angles of the RNA backbone (calculated using DSSR4) are compared against the statistical distribution of a benchmark set of high quality, non-redundant6 RNA structures determined by X-ray crystallography at ≤ 2.5 Å. Separate analyses are performed based on sugar-puckering type (of ~27,000 residues in the benchmark set, ~24,000 are C3'-endo-like, ~3,000 are C2'-endo-like). Output plots and and a downloadable (csv format) table identify probable conformational outliers for any given RNA-containing structure. For comparison, RNA Suiteness7,8 scores are also provided in the csv (for residue i, suiteness of the i-1, i dinucleotide is listed. Suiteness range is 0(poor)-1(excellent). Calculation performed using DSSR).
The 2D plots permit comparison of pseudotorsion pairs for the structure being evaluated (plotted points) against population statistics of the benchmark set (smoothed contours: bar at right indicates benchmark residue count range). Outliers are marked on the plots by red crosses with residue information available on mouse hover. Summary statistics are provided below each plot to indicate the number of residues evaluated and percent outliers. Note that pseudotorsion angles cannot be calculated for terminal residues or around breaks in the polymer chain, so these are excluded from evaluation.
This tool is designed to identify regions of unusual conformation in folded RNA. Outliers can be legitimate but less-common backbone conformations, or they may indicate modelling error.
It may take several minutes to complete calculations for large structures such as ribosomes.
Please direct any questions/suggestions to help@emdataresource.org
- Olson, W. K. Configurational statistics of polynucleotide chains. An updated virtual bond model to treat effects of base stacking. Macromolecules 13, 721-728 (1980).
- Wadley, L. M., Keating, K. S., Duarte, C. M. & Pyle, A. M. Evaluating and learning from RNA pseudotorsional space: quantitative validation of a reduced representation for RNA structure. J Mol Biol 372, 942-957, doi (2007).
- Keating, K. S., Humphris, E. L. & Pyle, A. M. A new way to see RNA. Q Rev Biophys 44, 433-466, doi (2011).
- Lu, X. J., Bussemaker, H. J. & Olson, W. K. DSSR: an integrated software tool for dissecting the spatial structure of RNA. Nucleic Acids Res 43, e142, doi (2015).
- Li, S., Olson, W. K., Lu, X. J. Web 3DNA 2.0 for the analysis, visualization, and modeling of 3D nucleic acid structures. Nucleic Acids Research 47, W26-W34 (2019). doi
- Leontis, N. B., & Zirbel, C. L. Nonredundant 3D Structure Datasets for RNA Knowledge Extraction and Benchmarking. In RNA 3D Structure Analysis and Prediction, N. Leontis & E. Westhof (Eds.), (Vol. 27, pp. 281-298). Springer Berlin Heidelberg. doi (2012).
- Richardson, J. S., Schneider, B., Murray, L. W., Kapral, G. J., Immormino, R.M., Headd, J. J., Richardson, D. C., Ham, D., Hershkovits, E., Williams, L. D., Keating, K. S., Pyle, A. M., Micallef, D., Westbrook, J., Berman, H. M. RNA backbone: consensus all-angle conformers and modular string nomenclature (an RNA Ontology Consortium contribution). RNA 14, 465-81, doi (2008).
- Chen, V. B., Arendall III, W. B., Headd, J. J., Keedy, D. A., Immormino, R. M., Kapral, G. J., Murray, L. W., Richardson, J. S., Richardson, D. C., MolProbity: all-atom structure validation for macromolecular crystallography, Acta Cryst D 66, 12-21, doi (2010)
RNA Pseudotorsion Plots for PDB entry 4v8j
Crystal structure of the bacterial ribosome ram mutation G347U. Deposited 2011-12-20 X-RAY DIFFRACTION resol 3.9 Å
7956 C3'-endo RNA residues with 174 outliers ( 2.19 % ) from Eta-Theta plot
1148 C2'-endo RNA residues with 109 outliers ( 9.49 % ) from Eta-Theta plot
7956 C3'-endo RNA residues with 165 outliers ( 2.07 % ) from Eta'-Theta' plot
1148 C2'-endo RNA residues with 95 outliers ( 8.28 % ) from Eta'-Theta' plot
7956 C3'-endo RNA residues with 135 outliers ( 1.7 % ) from Eta"-Theta" plot
1148 C2'-endo RNA residues with 93 outliers ( 8.1 % ) from Eta"-Theta" plot
List of Outliers
| Model Number | Chain | Residue Type | Residue Identifier | Eta | Theta | Eta' | Theta' | Eta'' | Theta'' | Sugar Pucker | Suiteness |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | AA | C | 84 | 102.3 | 318.0 | 128.1 | 325.1 | 126.9 | 359.7 | C3'endo | 0.0 |
| 1 | AA | C | 86 | 220.2 | 272.1 | 230.6 | 280.2 | --- | --- | C3'endo | 0.048 |
| 1 | AA | C | 347 | 120.2 | 70.7 | 99.7 | 62.1 | 102.8 | 336.0 | C3'endo | 0.0 |
| 1 | AA | A | 348 | 11.6 | 109.9 | 6.1 | 115.5 | --- | --- | C3'endo | 0.0 |
| 1 | AA | U | 363 | 278.2 | 334.9 | --- | --- | --- | --- | C3'endo | 0.198 |
| 1 | AA | A | 392 | 319.0 | 146.9 | 318.7 | 119.3 | 327.5 | 58.7 | C3'endo | 0.0 |
| 1 | AA | A | 515 | 314.4 | 294.1 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | AA | U | 706 | 73.0 | 289.8 | 75.4 | 286.2 | --- | --- | C3'endo | 0.0 |
| 1 | AA | A | 799 | 69.4 | 27.2 | 87.8 | 7.1 | --- | --- | C3'endo | 0.0 |
| 1 | AA | U | 822 | 34.6 | 32.2 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | AA | U | 824 | 342.1 | 323.4 | 354.9 | 319.7 | --- | --- | C3'endo | 0.0 |
| 1 | AA | A | 983 | 227.7 | 279.3 | 254.7 | 281.8 | --- | --- | C3'endo | 0.0 |
| 1 | AA | C | 1036 | 27.2 | 114.5 | 352.6 | 121.8 | 13.5 | 138.5 | C3'endo | 0.0 |
| 1 | AA | U | 1107 | 18.4 | 54.8 | 22.7 | 24.5 | 33.0 | 354.5 | C3'endo | 0.0 |
| 1 | AA | U | 1118 | 58.6 | 2.1 | 73.9 | 344.7 | 87.3 | 5.8 | C3'endo | 0.0 |
| 1 | AA | U | 1192 | 208.9 | 120.1 | --- | --- | --- | --- | C3'endo | 0.889 |
| 1 | AA | U | 1193 | 285.0 | 39.9 | 248.1 | 55.8 | --- | --- | C3'endo | 0.0 |
| 1 | AA | A | 1237 | 257.3 | 134.4 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | AA | U | 1259 | 229.8 | 38.5 | 235.3 | 37.1 | --- | --- | C3'endo | 0.0 |
| 1 | AA | G | 1281 | 320.2 | 33.3 | 284.9 | 81.1 | --- | --- | C3'endo | 0.0 |
| 1 | AA | C | 1379 | 337.3 | 61.8 | 335.8 | 36.0 | 183.7 | 34.5 | C3'endo | 0.01 |
| 1 | AA | A | 1380 | 128.7 | 46.3 | 143.6 | 70.0 | --- | --- | C3'endo | 0.0 |
| 1 | AA | A | 1431 | 69.5 | 23.7 | 81.4 | 327.2 | --- | --- | C3'endo | 0.0 |
| 1 | AW | G | 15 | 127.1 | 127.0 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | AV | U | 48 | 52.1 | 314.9 | 46.9 | 313.5 | 66.2 | 356.0 | C3'endo | 0.0 |
| 1 | BA | G | 100 | 45.0 | 344.7 | 41.6 | 348.9 | 92.1 | 355.3 | C3'endo | 0.447 |
| 1 | BA | G | 102 | 201.1 | 59.1 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | BA | A | 229 | 313.1 | 4.6 | 9.4 | 351.1 | 36.7 | 340.9 | C3'endo | 0.0 |
| 1 | BA | G | 248 | 122.9 | 300.3 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | BA | U | 272A | 6.8 | 353.4 | 359.0 | 347.7 | --- | --- | C3'endo | 0.0 |
| 1 | BA | A | 283 | 104.7 | 280.5 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | BA | A | 332 | 222.6 | 270.0 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | BA | G | 352 | 252.6 | 114.3 | 235.8 | 100.6 | --- | --- | C3'endo | 0.0 |
| 1 | BA | A | 443 | 271.3 | 85.9 | 312.6 | 67.3 | 16.3 | 13.3 | C3'endo | 0.648 |
| 1 | BA | A | 480 | 113.4 | 299.3 | --- | --- | --- | --- | C3'endo | 0.012 |
| 1 | BA | U | 504 | 325.9 | 89.1 | 326.4 | 58.9 | 310.3 | 2.9 | C3'endo | 0.4 |
| 1 | BA | G | 508 | 313.2 | 77.8 | 292.2 | 64.8 | --- | --- | C3'endo | 0.0 |
| 1 | BA | A | 572 | 54.7 | 260.9 | --- | --- | --- | --- | C3'endo | 0.518 |
| 1 | BA | C | 645 | 60.0 | 13.9 | 68.7 | 321.3 | --- | --- | C3'endo | 0.0 |
| 1 | BA | G | 729 | 269.9 | 24.9 | --- | --- | --- | --- | C3'endo | 0.01 |
| 1 | BA | U | 747 | 317.6 | 36.2 | 341.9 | 19.5 | 146.0 | 12.6 | C3'endo | 0.16 |
| 1 | BA | A | 751 | 301.7 | 106.1 | 322.7 | 120.9 | 318.5 | 122.4 | C3'endo | 0.877 |
| 1 | BA | U | 827 | 338.7 | 49.9 | 346.0 | 45.3 | 332.6 | 53.6 | C3'endo | 0.0 |
| 1 | BA | A | 887 | 257.6 | 130.2 | 249.8 | 120.1 | --- | --- | C3'endo | 0.027 |
| 1 | BA | U | 958 | 316.7 | 91.3 | 353.3 | 84.9 | --- | --- | C3'endo | 0.0 |
| 1 | BA | A | 1045 | 205.4 | 67.0 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | BA | A | 1046 | 5.3 | 10.1 | 12.3 | 317.4 | --- | --- | C3'endo | 0.039 |
| 1 | BA | C | 1135 | 76.7 | 267.6 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | BA | G | 1136 | 11.8 | 163.1 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | BA | A | 1177 | 268.4 | 86.9 | 287.9 | 73.6 | --- | --- | C3'endo | 0.014 |
| 1 | BA | U | 1273 | 336.9 | 143.5 | 29.8 | 116.3 | 64.4 | 84.7 | C3'endo | 0.1 |
| 1 | BA | U | 1300 | 311.4 | 71.6 | 281.4 | 64.1 | --- | --- | C3'endo | 0.0 |
| 1 | BA | G | 1324 | 198.8 | 311.1 | --- | --- | --- | --- | C3'endo | 0.159 |
| 1 | BA | U | 1420 | 11.3 | 311.8 | 63.6 | 287.0 | --- | --- | C3'endo | 0.0 |
| 1 | BA | U | 1497 | 121.4 | 284.6 | 98.1 | 302.2 | --- | --- | C3'endo | 0.0 |
| 1 | BA | C | 1509 | 291.4 | 332.6 | 271.8 | 343.0 | --- | --- | C3'endo | 0.0 |
| 1 | BA | C | 1543 | 32.4 | 307.6 | 36.1 | 285.6 | --- | --- | C3'endo | 0.124 |
| 1 | BA | U | 1757 | 328.7 | 355.4 | 327.2 | 335.7 | 21.6 | 350.8 | C3'endo | 0.45 |
| 1 | BA | A | 1819 | 214.2 | 109.6 | --- | --- | --- | --- | C3'endo | 0.256 |
| 1 | BA | C | 1965 | 266.4 | 345.5 | --- | --- | --- | --- | C3'endo | 0.062 |
| 1 | BA | A | 1971 | 2.4 | 343.8 | 352.6 | 334.9 | --- | --- | C3'endo | 0.0 |
| 1 | BA | A | 1981 | 308.1 | 276.7 | --- | --- | --- | --- | C3'endo | 0.899 |
| 1 | BA | A | 2030 | 7.7 | 106.3 | 35.7 | 101.4 | 19.0 | 76.8 | C3'endo | 0.46 |
| 1 | BA | C | 2055 | 235.7 | 47.5 | 256.4 | 58.7 | 305.5 | 125.6 | C3'endo | 0.0 |
| 1 | BA | U | 2132 | 320.9 | 19.8 | 351.5 | 348.6 | --- | --- | C3'endo | 0.046 |
| 1 | BA | G | 2206 | 108.7 | 289.7 | --- | --- | --- | --- | C3'endo | 0.01 |
| 1 | BA | U | 2218 | 21.1 | 322.6 | 44.3 | 306.8 | --- | --- | C3'endo | 0.049 |
| 1 | BA | A | 2267 | 126.2 | 30.7 | 142.3 | 26.9 | --- | --- | C3'endo | 0.687 |
| 1 | BA | G | 2282 | 203.0 | 39.5 | 211.7 | 26.1 | 196.3 | 41.1 | C3'endo | 0.866 |
| 1 | BA | C | 2424 | 92.0 | 333.0 | 92.4 | 322.5 | --- | --- | C3'endo | 0.033 |
| 1 | BA | G | 2428 | 291.1 | 100.7 | 274.6 | 95.1 | --- | --- | C3'endo | 0.041 |
| 1 | BA | G | 2502 | 338.9 | 38.0 | 359.4 | 37.0 | 137.2 | 61.0 | C3'endo | 0.0 |
| 1 | BA | G | 2505 | 346.8 | 137.0 | 345.3 | 131.4 | --- | --- | C3'endo | 0.0 |
| 1 | BA | C | 2573 | 265.1 | 62.3 | 255.4 | 65.1 | --- | --- | C3'endo | 0.0 |
| 1 | BA | U | 2585 | 111.0 | 104.4 | 101.8 | 129.7 | 53.6 | 180.8 | C3'endo | 0.0 |
| 1 | BA | U | 2689 | 104.9 | 58.7 | 124.7 | 62.2 | 161.9 | 84.9 | C3'endo | 0.0 |
| 1 | BA | A | 2712A | 81.7 | 329.0 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | BA | A | 2765 | 86.2 | 261.2 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | BA | G | 2893 | 202.6 | 20.5 | 227.5 | 38.0 | 280.8 | 72.6 | C3'endo | 0.0 |
| 1 | BB | U | 41 | 287.2 | 325.2 | 353.6 | 299.0 | --- | --- | C3'endo | 0.281 |
| 1 | BB | G | 44 | 260.1 | 130.9 | 297.9 | 91.3 | 302.1 | 75.0 | C3'endo | 0.37 |
| 1 | BB | C | 109 | 219.5 | 49.1 | 220.3 | 51.5 | --- | --- | C3'endo | 0.0 |
| 1 | CA | C | 84 | 91.4 | 315.1 | 121.0 | 321.1 | 129.2 | 351.1 | C3'endo | 0.0 |
| 1 | CA | U | 85 | 197.8 | 50.0 | 236.7 | 49.0 | --- | --- | C3'endo | 0.0 |
| 1 | CA | C | 86 | 235.0 | 285.9 | 251.6 | 290.2 | --- | --- | C3'endo | 0.038 |
| 1 | CA | C | 347 | 105.4 | 77.2 | 88.7 | 63.4 | 99.9 | 341.0 | C3'endo | 0.0 |
| 1 | CA | A | 348 | 4.5 | 114.0 | 358.3 | 119.9 | --- | --- | C3'endo | 0.0 |
| 1 | CA | U | 363 | 279.5 | 337.7 | --- | --- | --- | --- | C3'endo | 0.443 |
| 1 | CA | A | 515 | 324.0 | 286.9 | 290.0 | 277.8 | --- | --- | C3'endo | 0.0 |
| 1 | CA | U | 706 | 61.4 | 280.4 | 68.0 | 280.4 | --- | --- | C3'endo | 0.0 |
| 1 | CA | C | 737 | 131.0 | 299.9 | 104.1 | 315.3 | --- | --- | C3'endo | 0.0 |
| 1 | CA | A | 799 | 73.7 | 32.6 | --- | --- | --- | --- | C3'endo | 0.053 |
| 1 | CA | U | 822 | 57.5 | 8.7 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | CA | U | 824 | 320.5 | 334.6 | 322.6 | 327.4 | --- | --- | C3'endo | 0.0 |
| 1 | CA | G | 903 | 53.9 | 61.2 | 53.2 | 17.5 | --- | --- | C3'endo | 0.01 |
| 1 | CA | A | 983 | 223.8 | 281.1 | 253.7 | 283.1 | --- | --- | C3'endo | 0.0 |
| 1 | CA | C | 1036 | 26.0 | 106.9 | 354.4 | 116.3 | 17.5 | 142.6 | C3'endo | 0.0 |
| 1 | CA | U | 1107 | 30.4 | 35.7 | 26.2 | 15.7 | 40.7 | 347.8 | C3'endo | 0.0 |
| 1 | CA | U | 1192 | 217.2 | 104.8 | --- | --- | --- | --- | C3'endo | 0.808 |
| 1 | CA | A | 1237 | 262.2 | 130.0 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | CA | U | 1259 | 222.8 | 37.9 | 234.3 | 42.1 | --- | --- | C3'endo | 0.0 |
| 1 | CA | G | 1281 | 327.2 | 30.4 | 295.5 | 75.5 | --- | --- | C3'endo | 0.0 |
| 1 | CV | U | 48 | 40.0 | 296.6 | 41.7 | 300.4 | 63.4 | 355.7 | C3'endo | 0.0 |
| 1 | DA | A | 71 | 216.6 | 78.8 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | DA | G | 100 | 44.9 | 344.7 | 41.6 | 348.9 | 92.1 | 355.4 | C3'endo | 0.448 |
| 1 | DA | G | 102 | 201.1 | 59.2 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | DA | A | 229 | 313.2 | 4.6 | 9.4 | 351.1 | 36.7 | 340.9 | C3'endo | 0.0 |
| 1 | DA | G | 248 | 122.9 | 300.3 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | DA | U | 272A | 9.4 | 354.0 | 2.9 | 347.3 | --- | --- | C3'endo | 0.0 |
| 1 | DA | A | 283 | 104.7 | 280.4 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | DA | A | 332 | 222.7 | 270.0 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | DA | G | 352 | 252.6 | 114.4 | 235.8 | 100.6 | --- | --- | C3'endo | 0.0 |
| 1 | DA | A | 443 | 271.3 | 85.9 | 312.6 | 67.3 | 16.3 | 13.3 | C3'endo | 0.648 |
| 1 | DA | A | 480 | 113.3 | 299.2 | --- | --- | --- | --- | C3'endo | 0.012 |
| 1 | DA | U | 504 | 325.9 | 89.1 | 326.4 | 58.9 | 310.3 | 2.9 | C3'endo | 0.402 |
| 1 | DA | G | 508 | 313.2 | 77.7 | 292.2 | 64.7 | --- | --- | C3'endo | 0.0 |
| 1 | DA | A | 572 | 54.7 | 260.9 | --- | --- | --- | --- | C3'endo | 0.518 |
| 1 | DA | C | 645 | 59.9 | 13.9 | 68.7 | 321.3 | --- | --- | C3'endo | 0.0 |
| 1 | DA | G | 729 | 269.9 | 24.9 | --- | --- | --- | --- | C3'endo | 0.01 |
| 1 | DA | U | 747 | 317.5 | 36.2 | 341.9 | 19.5 | 146.0 | 12.6 | C3'endo | 0.16 |
| 1 | DA | A | 751 | 301.7 | 106.1 | 322.7 | 120.9 | 318.5 | 122.4 | C3'endo | 0.875 |
| 1 | DA | U | 827 | 338.8 | 49.8 | 346.0 | 45.3 | 332.6 | 53.6 | C3'endo | 0.0 |
| 1 | DA | A | 887 | 257.6 | 130.2 | 249.8 | 120.2 | --- | --- | C3'endo | 0.027 |
| 1 | DA | U | 958 | 316.8 | 91.3 | 353.3 | 85.0 | --- | --- | C3'endo | 0.0 |
| 1 | DA | A | 1045 | 205.4 | 67.0 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | DA | A | 1046 | 5.2 | 10.1 | 12.3 | 317.4 | --- | --- | C3'endo | 0.039 |
| 1 | DA | C | 1135 | 76.7 | 267.6 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | DA | G | 1136 | 11.9 | 163.1 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | DA | A | 1177 | 268.4 | 86.9 | 287.9 | 73.7 | --- | --- | C3'endo | 0.014 |
| 1 | DA | U | 1273 | 336.9 | 143.5 | 29.8 | 116.3 | 64.4 | 84.7 | C3'endo | 0.1 |
| 1 | DA | U | 1300 | 311.3 | 71.7 | 281.4 | 64.1 | --- | --- | C3'endo | 0.0 |
| 1 | DA | G | 1324 | 198.8 | 311.2 | --- | --- | --- | --- | C3'endo | 0.16 |
| 1 | DA | U | 1420 | 11.3 | 311.8 | 63.6 | 287.1 | --- | --- | C3'endo | 0.0 |
| 1 | DA | U | 1497 | 121.4 | 284.6 | 98.1 | 302.2 | --- | --- | C3'endo | 0.0 |
| 1 | DA | C | 1509 | 291.4 | 332.6 | 271.8 | 343.0 | --- | --- | C3'endo | 0.0 |
| 1 | DA | C | 1543 | 28.1 | 313.3 | 34.0 | 288.7 | --- | --- | C3'endo | 0.11 |
| 1 | DA | U | 1757 | 328.7 | 355.4 | 327.2 | 335.7 | 21.5 | 350.8 | C3'endo | 0.449 |
| 1 | DA | C | 1965 | 266.4 | 345.5 | --- | --- | --- | --- | C3'endo | 0.061 |
| 1 | DA | A | 1971 | 2.5 | 343.8 | 352.6 | 334.9 | --- | --- | C3'endo | 0.0 |
| 1 | DA | A | 1981 | 308.1 | 276.7 | --- | --- | --- | --- | C3'endo | 0.899 |
| 1 | DA | A | 2030 | 7.7 | 106.3 | 35.7 | 101.4 | 19.0 | 76.8 | C3'endo | 0.46 |
| 1 | DA | C | 2055 | 235.7 | 47.5 | 256.4 | 58.7 | 305.5 | 125.6 | C3'endo | 0.0 |
| 1 | DA | U | 2132 | 320.9 | 19.9 | 351.5 | 348.7 | --- | --- | C3'endo | 0.046 |
| 1 | DA | G | 2206 | 108.7 | 289.7 | --- | --- | --- | --- | C3'endo | 0.01 |
| 1 | DA | U | 2218 | 21.2 | 322.6 | 44.3 | 306.8 | --- | --- | C3'endo | 0.049 |
| 1 | DA | A | 2267 | 126.2 | 30.7 | 142.3 | 26.9 | --- | --- | C3'endo | 0.684 |
| 1 | DA | G | 2282 | 203.0 | 39.5 | 211.7 | 26.1 | 196.3 | 41.1 | C3'endo | 0.866 |
| 1 | DA | C | 2424 | 94.6 | 333.7 | 94.8 | 325.0 | --- | --- | C3'endo | 0.0 |
| 1 | DA | G | 2428 | 291.1 | 100.7 | 274.6 | 95.1 | --- | --- | C3'endo | 0.041 |
| 1 | DA | G | 2502 | 338.9 | 38.0 | 359.3 | 37.0 | 137.2 | 61.0 | C3'endo | 0.0 |
| 1 | DA | G | 2505 | 346.8 | 137.0 | 345.3 | 131.4 | --- | --- | C3'endo | 0.0 |
| 1 | DA | C | 2573 | 265.1 | 62.3 | 255.4 | 65.1 | --- | --- | C3'endo | 0.0 |
| 1 | DA | U | 2585 | 111.0 | 104.4 | 101.8 | 129.7 | 53.6 | 180.8 | C3'endo | 0.0 |
| 1 | DA | U | 2689 | 104.9 | 58.7 | 124.7 | 62.1 | 161.9 | 84.9 | C3'endo | 0.0 |
| 1 | DA | A | 2712A | 81.7 | 329.0 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | DA | A | 2765 | 86.2 | 261.2 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | DA | G | 2893 | 202.6 | 20.5 | 227.5 | 38.0 | 280.8 | 72.6 | C3'endo | 0.0 |
| 1 | DB | U | 41 | 287.3 | 325.1 | 353.6 | 299.0 | --- | --- | C3'endo | 0.28 |
| 1 | DB | G | 44 | 260.1 | 130.9 | 297.9 | 91.3 | 302.1 | 75.0 | C3'endo | 0.371 |
| 1 | DB | C | 109 | 213.9 | 43.6 | 215.7 | 49.3 | --- | --- | C3'endo | 0.065 |
| 1 | AA | U | 85 | --- | --- | 229.1 | 63.4 | --- | --- | C3'endo | 0.0 |
| 1 | AA | G | 101 | --- | --- | 210.6 | 323.1 | --- | --- | C3'endo | 0.074 |
| 1 | AA | A | 705 | --- | --- | 125.3 | 127.0 | 149.9 | 63.0 | C3'endo | 0.758 |
| 1 | AA | G | 1171 | --- | --- | 224.3 | 120.8 | --- | --- | C3'endo | 0.0 |
| 1 | AA | C | 1344 | --- | --- | 127.4 | 4.5 | --- | --- | C3'endo | 0.0 |
| 1 | AW | A | 21 | --- | --- | 284.5 | 159.3 | --- | --- | C3'endo | 0.0 |
| 1 | AV | A | 22 | --- | --- | 271.1 | 153.2 | --- | --- | C3'endo | 0.0 |
| 1 | BA | U | 271Y | --- | --- | 152.3 | 96.6 | 133.2 | 94.9 | C3'endo | 0.0 |
| 1 | BA | A | 363F | --- | --- | 225.4 | 84.0 | --- | --- | C3'endo | 0.0 |
| 1 | BA | A | 781 | --- | --- | 349.3 | 83.6 | 338.7 | 22.8 | C3'endo | 0.147 |
| 1 | BA | G | 1112 | --- | --- | 270.0 | 170.0 | --- | --- | C3'endo | 0.0 |
| 1 | BA | G | 1173 | --- | --- | 277.3 | 260.9 | 327.4 | 311.2 | C3'endo | 0.0 |
| 1 | BA | A | 1174 | --- | --- | 226.8 | 73.9 | --- | --- | C3'endo | 0.0 |
| 1 | BA | A | 1542 | --- | --- | 117.3 | 137.4 | 117.1 | 61.7 | C3'endo | 0.0 |
| 1 | BA | U | 1779 | --- | --- | 152.1 | 29.6 | 199.0 | 340.6 | C3'endo | 0.0 |
| 1 | BA | G | 2116 | --- | --- | 6.7 | 272.6 | --- | --- | C3'endo | 0.0 |
| 1 | BA | G | 2131 | --- | --- | 134.0 | 50.3 | --- | --- | C3'endo | 0.0 |
| 1 | BA | G | 2133 | --- | --- | 224.9 | 118.0 | --- | --- | C3'endo | 0.0 |
| 1 | BA | G | 2207 | --- | --- | 330.5 | 127.8 | 15.2 | 44.0 | C3'endo | 0.0 |
| 1 | BA | A | 2208 | --- | --- | 89.5 | 118.3 | 100.0 | 27.6 | C3'endo | 0.0 |
| 1 | BA | A | 2577 | --- | --- | 281.1 | 355.4 | --- | --- | C3'endo | 0.254 |
| 1 | BA | C | 2755 | --- | --- | 350.6 | 148.6 | 354.0 | 95.2 | C3'endo | 0.038 |
| 1 | BA | G | 2777 | --- | --- | 321.4 | 68.0 | 265.0 | 14.5 | C3'endo | 0.525 |
| 1 | BB | A | 66 | --- | --- | 231.4 | 118.4 | --- | --- | C3'endo | 0.556 |
| 1 | CA | G | 261 | --- | --- | 348.5 | 82.1 | 310.4 | 48.0 | C3'endo | 0.0 |
| 1 | CA | A | 705 | --- | --- | 125.9 | 133.6 | 150.8 | 63.6 | C3'endo | 0.807 |
| 1 | CA | U | 1193 | --- | --- | 247.4 | 39.3 | --- | --- | C3'endo | 0.0 |
| 1 | CA | A | 1206 | --- | --- | 228.9 | 274.6 | --- | --- | C3'endo | 0.0 |
| 1 | CA | C | 1344 | --- | --- | 127.5 | 0.8 | --- | --- | C3'endo | 0.308 |
| 1 | CA | A | 1380 | --- | --- | 149.7 | 86.6 | --- | --- | C3'endo | 0.0 |
| 1 | CA | A | 1431 | --- | --- | 72.4 | 338.5 | --- | --- | C3'endo | 0.0 |
| 1 | CW | A | 21 | --- | --- | 288.5 | 156.5 | 296.9 | 146.7 | C3'endo | 0.0 |
| 1 | CV | A | 22 | --- | --- | 274.2 | 162.4 | --- | --- | C3'endo | 0.0 |
| 1 | DA | U | 271Y | --- | --- | 152.2 | 96.8 | 133.1 | 95.8 | C3'endo | 0.0 |
| 1 | DA | A | 363F | --- | --- | 225.4 | 83.9 | --- | --- | C3'endo | 0.0 |
| 1 | DA | A | 781 | --- | --- | 349.2 | 83.7 | 338.7 | 22.8 | C3'endo | 0.148 |
| 1 | DA | G | 1112 | --- | --- | 270.0 | 170.0 | --- | --- | C3'endo | 0.0 |
| 1 | DA | G | 1173 | --- | --- | 277.3 | 261.0 | 327.4 | 311.2 | C3'endo | 0.0 |
| 1 | DA | A | 1174 | --- | --- | 226.8 | 73.9 | --- | --- | C3'endo | 0.0 |
| 1 | DA | A | 1542 | --- | --- | 117.3 | 138.1 | 116.8 | 62.0 | C3'endo | 0.0 |
| 1 | DA | U | 1779 | --- | --- | 152.1 | 29.6 | 199.0 | 340.6 | C3'endo | 0.0 |
| 1 | DA | G | 2116 | --- | --- | 6.7 | 272.6 | --- | --- | C3'endo | 0.0 |
| 1 | DA | G | 2131 | --- | --- | 134.0 | 50.3 | --- | --- | C3'endo | 0.0 |
| 1 | DA | G | 2133 | --- | --- | 224.9 | 118.0 | --- | --- | C3'endo | 0.0 |
| 1 | DA | G | 2207 | --- | --- | 330.6 | 127.8 | 15.2 | 44.0 | C3'endo | 0.0 |
| 1 | DA | A | 2208 | --- | --- | 89.5 | 118.3 | 100.0 | 27.6 | C3'endo | 0.0 |
| 1 | DA | A | 2577 | --- | --- | 281.1 | 355.4 | --- | --- | C3'endo | 0.254 |
| 1 | DA | C | 2755 | --- | --- | 350.6 | 148.5 | 354.0 | 95.2 | C3'endo | 0.038 |
| 1 | DA | G | 2777 | --- | --- | 321.4 | 68.0 | 265.0 | 14.5 | C3'endo | 0.526 |
| 1 | DB | A | 66 | --- | --- | 231.4 | 118.3 | --- | --- | C3'endo | 0.557 |
| 1 | AA | A | 648 | --- | --- | --- | --- | 19.2 | 161.6 | C3'endo | 0.0 |
| 1 | AA | A | 954 | --- | --- | --- | --- | 70.8 | 181.1 | C3'endo | 0.0 |
| 1 | AA | G | 1004 | --- | --- | --- | --- | 161.3 | 330.4 | C3'endo | 0.047 |
| 1 | AA | C | 1005 | --- | --- | --- | --- | 298.4 | 177.9 | C3'endo | 0.0 |
| 1 | AA | C | 1010 | --- | --- | --- | --- | 349.2 | 314.6 | C3'endo | 0.0 |
| 1 | AA | A | 1164 | 355.3 | 289.1 | --- | --- | 65.5 | 177.3 | C3'endo | 0.195 |
| 1 | AA | G | 1183 | --- | --- | --- | --- | 331.0 | 294.1 | C3'endo | 0.0 |
| 1 | AA | U | 1238 | 316.3 | 357.8 | --- | --- | 288.5 | 87.0 | C3'endo | 0.0 |
| 1 | AA | A | 1469 | --- | --- | --- | --- | 34.4 | 189.5 | C3'endo | 0.0 |
| 1 | BA | A | 71 | 214.4 | 83.3 | --- | --- | 182.9 | 354.5 | C3'endo | 0.0 |
| 1 | BA | A | 221 | 223.3 | 37.0 | --- | --- | 159.9 | 82.2 | C3'endo | 0.0 |
| 1 | BA | G | 388 | --- | --- | --- | --- | 71.3 | 145.9 | C3'endo | 0.0 |
| 1 | BA | G | 614B | --- | --- | --- | --- | 296.1 | 80.4 | C3'endo | 0.0 |
| 1 | BA | G | 763 | --- | --- | --- | --- | 109.4 | 119.0 | C3'endo | 0.632 |
| 1 | BA | U | 1033 | --- | --- | --- | --- | 0.1 | 123.7 | C3'endo | 0.01 |
| 1 | BA | C | 1043 | --- | --- | --- | --- | 303.7 | 140.3 | C3'endo | 0.358 |
| 1 | BA | C | 1052 | --- | --- | --- | --- | 47.1 | 186.6 | C3'endo | 0.017 |
| 1 | BA | A | 1210 | 211.3 | 50.6 | --- | --- | 148.3 | 331.5 | C3'endo | 0.0 |
| 1 | BA | A | 1274 | --- | --- | --- | --- | 359.7 | 138.7 | C3'endo | 0.238 |
| 1 | BA | A | 1494 | --- | --- | --- | --- | 165.1 | 47.1 | C3'endo | 0.0 |
| 1 | BA | C | 1646 | --- | --- | --- | --- | 193.0 | 30.3 | C3'endo | 0.607 |
| 1 | BA | A | 1700 | --- | --- | --- | --- | 344.5 | 297.4 | C3'endo | 0.0 |
| 1 | BA | A | 1785 | --- | --- | --- | --- | 127.2 | 92.8 | C3'endo | 0.613 |
| 1 | BA | G | 1816 | 313.7 | 13.9 | --- | --- | 297.2 | 73.0 | C3'endo | 0.0 |
| 1 | BA | G | 2307 | --- | --- | --- | --- | 299.7 | 169.7 | C3'endo | 0.0 |
| 1 | BA | G | 2308 | --- | --- | --- | --- | 43.0 | 129.4 | C3'endo | 0.0 |
| 1 | BA | A | 2309 | --- | --- | --- | --- | 35.5 | 152.8 | C3'endo | 0.0 |
| 1 | BA | A | 2476 | --- | --- | --- | --- | 354.5 | 136.0 | C3'endo | 0.0 |
| 1 | BA | G | 2529 | --- | --- | --- | --- | 36.2 | 339.7 | C3'endo | 0.0 |
| 1 | BA | G | 2795 | --- | --- | --- | --- | 135.9 | 110.7 | C3'endo | 0.603 |
| 1 | BA | G | 2872 | --- | --- | --- | --- | 82.6 | 127.0 | C3'endo | 0.0 |
| 1 | BA | A | 2883 | --- | --- | --- | --- | 161.2 | 348.7 | C3'endo | 0.656 |
| 1 | CA | G | 6 | --- | --- | --- | --- | 130.5 | 102.4 | C3'endo | 0.043 |
| 1 | CA | U | 79 | --- | --- | --- | --- | 129.8 | 122.2 | C3'endo | 0.0 |
| 1 | CA | A | 392 | 324.3 | 149.9 | --- | --- | 332.5 | 66.7 | C3'endo | 0.0 |
| 1 | CA | A | 648 | --- | --- | --- | --- | 17.1 | 157.2 | C3'endo | 0.0 |
| 1 | CA | A | 954 | --- | --- | --- | --- | 76.4 | 178.3 | C3'endo | 0.0 |
| 1 | CA | G | 1004 | --- | --- | --- | --- | 159.3 | 332.5 | C3'endo | 0.0 |
| 1 | CA | C | 1010 | 314.9 | 269.6 | --- | --- | 357.4 | 309.8 | C3'endo | 0.024 |
| 1 | CA | U | 1118 | 59.4 | 13.9 | --- | --- | 81.8 | 8.1 | C3'endo | 0.0 |
| 1 | CA | A | 1164 | 12.7 | 281.8 | --- | --- | 45.7 | 182.5 | C3'endo | 0.01 |
| 1 | CA | G | 1183 | --- | --- | --- | --- | 313.3 | 296.8 | C3'endo | 0.0 |
| 1 | CA | U | 1238 | 317.9 | 358.6 | --- | --- | 288.3 | 82.7 | C3'endo | 0.0 |
| 1 | CA | A | 1469 | --- | --- | --- | --- | 40.3 | 198.0 | C3'endo | 0.0 |
| 1 | DA | A | 221 | 223.3 | 37.0 | --- | --- | 159.9 | 82.2 | C3'endo | 0.0 |
| 1 | DA | G | 388 | --- | --- | --- | --- | 71.3 | 145.9 | C3'endo | 0.0 |
| 1 | DA | G | 614B | --- | --- | --- | --- | 296.1 | 80.4 | C3'endo | 0.0 |
| 1 | DA | G | 763 | --- | --- | --- | --- | 109.4 | 119.0 | C3'endo | 0.636 |
| 1 | DA | U | 1033 | --- | --- | --- | --- | 0.1 | 123.7 | C3'endo | 0.01 |
| 1 | DA | C | 1043 | --- | --- | --- | --- | 303.7 | 140.3 | C3'endo | 0.36 |
| 1 | DA | C | 1052 | --- | --- | --- | --- | 47.1 | 186.7 | C3'endo | 0.017 |
| 1 | DA | A | 1210 | 211.3 | 50.6 | --- | --- | 148.3 | 331.5 | C3'endo | 0.0 |
| 1 | DA | A | 1274 | --- | --- | --- | --- | 359.7 | 138.6 | C3'endo | 0.238 |
| 1 | DA | A | 1494 | --- | --- | --- | --- | 165.2 | 47.1 | C3'endo | 0.0 |
| 1 | DA | C | 1646 | --- | --- | --- | --- | 193.0 | 30.3 | C3'endo | 0.606 |
| 1 | DA | A | 1700 | --- | --- | --- | --- | 344.5 | 297.4 | C3'endo | 0.0 |
| 1 | DA | A | 1785 | --- | --- | --- | --- | 127.2 | 92.8 | C3'endo | 0.612 |
| 1 | DA | G | 1816 | 313.7 | 13.9 | --- | --- | 297.2 | 73.0 | C3'endo | 0.0 |
| 1 | DA | G | 2307 | --- | --- | --- | --- | 300.0 | 169.7 | C3'endo | 0.0 |
| 1 | DA | G | 2308 | --- | --- | --- | --- | 43.0 | 129.4 | C3'endo | 0.0 |
| 1 | DA | A | 2309 | --- | --- | --- | --- | 35.4 | 152.7 | C3'endo | 0.0 |
| 1 | DA | A | 2476 | --- | --- | --- | --- | 354.5 | 136.0 | C3'endo | 0.0 |
| 1 | DA | G | 2529 | --- | --- | --- | --- | 36.2 | 339.7 | C3'endo | 0.0 |
| 1 | DA | G | 2795 | --- | --- | --- | --- | 135.9 | 110.7 | C3'endo | 0.605 |
| 1 | DA | G | 2872 | --- | --- | --- | --- | 82.6 | 127.0 | C3'endo | 0.0 |
| 1 | DA | A | 2883 | --- | --- | --- | --- | 161.2 | 348.7 | C3'endo | 0.657 |
| 1 | AA | A | 51 | 27.5 | 59.9 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | AA | A | 60 | 279.0 | 198.0 | 34.0 | 131.4 | --- | --- | C2'endo | 0.01 |
| 1 | AA | G | 190 | 344.8 | 47.6 | 99.9 | 351.3 | --- | --- | C2'endo | 0.0 |
| 1 | AA | A | 339 | 70.8 | 358.6 | 86.8 | 323.5 | --- | --- | C2'endo | 0.0 |
| 1 | AA | G | 468 | 24.7 | 189.9 | --- | --- | --- | --- | C2'endo | 0.095 |
| 1 | AA | C | 731 | 114.0 | 193.0 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | AA | A | 798 | 68.9 | 28.0 | 60.7 | 353.6 | --- | --- | C2'endo | 0.01 |
| 1 | AA | U | 803 | 164.5 | 7.5 | --- | --- | --- | --- | C2'endo | 0.337 |
| 1 | AA | A | 951 | 61.3 | 74.7 | 115.3 | 33.2 | --- | --- | C2'endo | 0.0 |
| 1 | AA | A | 952 | 34.6 | 62.1 | 36.7 | 65.5 | 59.3 | 74.5 | C2'endo | 0.0 |
| 1 | AA | U | 969 | 354.0 | 65.6 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | AA | U | 1031 | 18.7 | 75.1 | 25.1 | 37.3 | 0.7 | 31.0 | C2'endo | 0.44 |
| 1 | AA | G | 1076 | 25.9 | 51.4 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | AA | C | 1432 | 126.6 | 105.5 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | AA | A | 1479 | 145.8 | 280.7 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | AW | G | 19 | 250.8 | 285.1 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | BA | U | 99 | 189.1 | 31.2 | --- | --- | --- | --- | C2'endo | 0.078 |
| 1 | BA | G | 386 | 106.3 | 328.9 | 120.3 | 310.8 | --- | --- | C2'endo | 0.0 |
| 1 | BA | A | 532 | 145.9 | 30.1 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | BA | G | 573 | 245.9 | 198.9 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | BA | C | 574 | 26.5 | 86.5 | 63.6 | 62.0 | --- | --- | C2'endo | 0.289 |
| 1 | BA | C | 791 | 258.9 | 206.6 | 286.5 | 181.3 | --- | --- | C2'endo | 0.0 |
| 1 | BA | G | 859 | 127.5 | 167.5 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | BA | A | 945 | 102.9 | 227.8 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | BA | G | 974 | 66.2 | 23.6 | 112.8 | 357.9 | --- | --- | C2'endo | 0.0 |
| 1 | BA | C | 975 | 67.7 | 12.8 | 60.7 | 18.3 | --- | --- | C2'endo | 0.0 |
| 1 | BA | U | 1012 | 283.1 | 21.1 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | BA | G | 1025 | 34.3 | 59.0 | 103.4 | 23.4 | --- | --- | C2'endo | 0.0 |
| 1 | BA | A | 1048 | 248.4 | 214.1 | 277.3 | 138.1 | --- | --- | C2'endo | 0.033 |
| 1 | BA | A | 1126 | 80.6 | 89.0 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | BA | U | 1175 | 93.7 | 76.7 | 93.8 | 55.3 | --- | --- | C2'endo | 0.0 |
| 1 | BA | G | 1176 | 82.7 | 235.7 | 75.2 | 235.3 | --- | --- | C2'endo | 0.0 |
| 1 | BA | G | 1252 | 11.7 | 232.5 | 44.8 | 202.5 | --- | --- | C2'endo | 0.486 |
| 1 | BA | G | 1385 | 140.4 | 95.1 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | BA | G | 1416 | 325.5 | 120.5 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | BA | A | 1722 | 23.8 | 253.5 | 81.0 | 189.7 | 28.3 | 90.0 | C2'endo | 0.0 |
| 1 | BA | G | 1758 | 242.6 | 280.0 | 279.4 | 279.7 | --- | --- | C2'endo | 0.606 |
| 1 | BA | A | 1913 | 321.3 | 134.9 | 48.6 | 95.0 | --- | --- | C2'endo | 0.061 |
| 1 | BA | G | 1930 | 145.4 | 73.6 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | BA | A | 1938 | 18.1 | 254.8 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | BA | U | 1963 | 35.8 | 218.3 | 40.4 | 187.0 | --- | --- | C2'endo | 0.0 |
| 1 | BA | G | 1992 | 304.2 | 135.3 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | BA | A | 2031 | 117.8 | 65.1 | 128.3 | 355.9 | --- | --- | C2'endo | 0.0 |
| 1 | BA | U | 2172 | 150.1 | 5.7 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | BA | A | 2266 | 58.3 | 78.7 | --- | --- | --- | --- | C2'endo | 0.595 |
| 1 | BA | A | 2311 | 259.6 | 272.8 | 297.6 | 264.2 | --- | --- | C2'endo | 0.0 |
| 1 | BA | A | 2346 | 333.5 | 51.0 | 339.4 | 6.6 | 320.4 | 351.2 | C2'endo | 0.125 |
| 1 | BA | A | 2425 | 104.4 | 64.8 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | BA | A | 2602 | 73.2 | 32.6 | 41.2 | 31.9 | --- | --- | C2'endo | 0.0 |
| 1 | BA | A | 2713 | 52.8 | 240.8 | 85.0 | 215.1 | --- | --- | C2'endo | 0.0 |
| 1 | BA | G | 2867 | 338.6 | 48.6 | --- | --- | --- | --- | C2'endo | 0.432 |
| 1 | CA | A | 51 | 21.1 | 56.6 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | CA | A | 60 | 276.7 | 188.7 | 31.7 | 126.5 | --- | --- | C2'endo | 0.0 |
| 1 | CA | G | 190 | 2.0 | 28.2 | 107.9 | 346.0 | --- | --- | C2'endo | 0.0 |
| 1 | CA | A | 339 | 75.6 | 358.8 | 82.7 | 333.0 | --- | --- | C2'endo | 0.0 |
| 1 | CA | C | 340 | 153.6 | 67.7 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | CA | G | 468 | 25.0 | 193.8 | --- | --- | --- | --- | C2'endo | 0.274 |
| 1 | CA | A | 798 | 73.3 | 19.1 | 55.5 | 2.7 | --- | --- | C2'endo | 0.0 |
| 1 | CA | U | 803 | 160.9 | 356.5 | --- | --- | --- | --- | C2'endo | 0.072 |
| 1 | CA | A | 951 | 97.9 | 49.5 | 121.0 | 33.0 | --- | --- | C2'endo | 0.0 |
| 1 | CA | A | 952 | 28.0 | 74.6 | 34.9 | 66.9 | 62.7 | 68.7 | C2'endo | 0.0 |
| 1 | CA | U | 1031 | 17.1 | 73.5 | 23.9 | 37.1 | --- | --- | C2'endo | 0.555 |
| 1 | CA | G | 1046 | 3.9 | 190.5 | --- | --- | --- | --- | C2'endo | 0.01 |
| 1 | CA | G | 1076 | 15.3 | 65.5 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | CA | G | 1286 | 132.5 | 187.8 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | CA | A | 1479 | 149.3 | 283.9 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | CW | C | 17 | 7.2 | 41.6 | 31.8 | 54.5 | 25.9 | 132.3 | C2'endo | 0.0 |
| 1 | CW | G | 18 | 276.1 | 224.1 | 285.9 | 231.3 | --- | --- | C2'endo | 0.0 |
| 1 | CW | G | 19 | 272.0 | 288.5 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | CV | G | 19 | 298.3 | 224.1 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | DA | U | 99 | 189.1 | 31.2 | --- | --- | --- | --- | C2'endo | 0.078 |
| 1 | DA | G | 386 | 106.4 | 328.9 | 120.3 | 310.8 | --- | --- | C2'endo | 0.0 |
| 1 | DA | A | 532 | 145.8 | 30.1 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | DA | G | 573 | 245.9 | 198.9 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | DA | C | 574 | 26.5 | 86.5 | 63.6 | 62.0 | --- | --- | C2'endo | 0.289 |
| 1 | DA | C | 791 | 258.9 | 206.6 | 286.5 | 181.3 | --- | --- | C2'endo | 0.0 |
| 1 | DA | G | 859 | 127.6 | 167.5 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | DA | A | 945 | 102.9 | 227.8 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | DA | G | 974 | 66.2 | 23.6 | 112.8 | 357.9 | --- | --- | C2'endo | 0.0 |
| 1 | DA | C | 975 | 67.7 | 12.8 | 60.7 | 18.3 | --- | --- | C2'endo | 0.0 |
| 1 | DA | U | 1012 | 283.1 | 21.1 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | DA | G | 1025 | 34.3 | 58.9 | 103.4 | 23.4 | --- | --- | C2'endo | 0.0 |
| 1 | DA | A | 1048 | 248.3 | 214.2 | 277.3 | 138.1 | --- | --- | C2'endo | 0.033 |
| 1 | DA | A | 1126 | 80.6 | 89.0 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | DA | U | 1175 | 93.7 | 76.7 | 93.8 | 55.3 | --- | --- | C2'endo | 0.0 |
| 1 | DA | G | 1176 | 82.7 | 235.7 | 75.2 | 235.3 | --- | --- | C2'endo | 0.0 |
| 1 | DA | G | 1252 | 11.7 | 232.5 | 44.8 | 202.5 | --- | --- | C2'endo | 0.486 |
| 1 | DA | G | 1416 | 325.5 | 120.5 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | DA | A | 1722 | 23.8 | 253.5 | 81.0 | 189.7 | 28.3 | 90.0 | C2'endo | 0.0 |
| 1 | DA | G | 1758 | 242.6 | 280.0 | 279.4 | 279.7 | --- | --- | C2'endo | 0.607 |
| 1 | DA | A | 1913 | 321.3 | 134.9 | 48.6 | 95.1 | --- | --- | C2'endo | 0.061 |
| 1 | DA | G | 1930 | 145.4 | 73.6 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | DA | A | 1938 | 18.1 | 254.7 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | DA | U | 1963 | 35.9 | 218.4 | 40.5 | 187.1 | --- | --- | C2'endo | 0.0 |
| 1 | DA | A | 2031 | 117.8 | 65.1 | 128.3 | 356.0 | --- | --- | C2'endo | 0.0 |
| 1 | DA | U | 2172 | 150.0 | 5.8 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | DA | A | 2266 | 58.4 | 78.7 | --- | --- | --- | --- | C2'endo | 0.597 |
| 1 | DA | A | 2311 | 259.6 | 272.8 | 297.6 | 264.2 | --- | --- | C2'endo | 0.0 |
| 1 | DA | A | 2346 | 333.4 | 51.0 | 339.4 | 6.6 | 320.4 | 351.2 | C2'endo | 0.125 |
| 1 | DA | A | 2425 | 105.2 | 64.8 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | DA | A | 2602 | 73.2 | 32.6 | 41.2 | 31.9 | --- | --- | C2'endo | 0.0 |
| 1 | DA | A | 2713 | 52.8 | 240.8 | 85.0 | 215.1 | --- | --- | C2'endo | 0.0 |
| 1 | DA | G | 2867 | 338.6 | 48.7 | --- | --- | --- | --- | C2'endo | 0.433 |
| 1 | AA | A | 8 | --- | --- | 278.4 | 246.4 | 299.9 | 266.3 | C2'endo | 0.138 |
| 1 | AA | A | 50 | --- | --- | 74.7 | 96.7 | 84.5 | 96.2 | C2'endo | 0.129 |
| 1 | AA | G | 90 | --- | --- | 171.9 | 192.8 | --- | --- | C2'endo | 0.0 |
| 1 | AA | A | 102 | --- | --- | 148.1 | 289.3 | --- | --- | C2'endo | 0.432 |
| 1 | AA | U | 189 | --- | --- | 328.3 | 248.7 | 315.7 | 214.2 | C2'endo | 0.039 |
| 1 | AA | A | 445 | --- | --- | 81.7 | 110.1 | --- | --- | C2'endo | 0.272 |
| 1 | AA | A | 454 | --- | --- | 67.8 | 190.8 | 33.9 | 147.8 | C2'endo | 0.0 |
| 1 | AA | A | 480 | --- | --- | 154.2 | 208.9 | --- | --- | C2'endo | 0.0 |
| 1 | AA | C | 684 | --- | --- | 173.6 | 8.0 | --- | --- | C2'endo | 0.57 |
| 1 | AA | C | 823 | --- | --- | 167.0 | 122.1 | --- | --- | C2'endo | 0.0 |
| 1 | AA | U | 1177 | --- | --- | 291.9 | 32.6 | --- | --- | C2'endo | 0.0 |
| 1 | AA | A | 1182 | --- | --- | 36.1 | 84.3 | 89.4 | 81.7 | C2'endo | 0.126 |
| 1 | AA | A | 1345 | --- | --- | 64.8 | 67.7 | --- | --- | C2'endo | 0.0 |
| 1 | AW | C | 48 | --- | --- | 358.8 | 13.4 | --- | --- | C2'endo | 0.0 |
| 1 | BA | A | 196 | --- | --- | 327.7 | 245.1 | --- | --- | C2'endo | 0.0 |
| 1 | BA | A | 265 | --- | --- | 79.8 | 153.3 | --- | --- | C2'endo | 0.05 |
| 1 | BA | U | 271N | --- | --- | 12.4 | 354.6 | 344.7 | 34.2 | C2'endo | 0.0 |
| 1 | BA | A | 330 | --- | --- | 149.4 | 199.2 | --- | --- | C2'endo | 0.0 |
| 1 | BA | A | 457 | --- | --- | 8.8 | 150.5 | 18.1 | 154.0 | C2'endo | 0.0 |
| 1 | BA | G | 530 | --- | --- | 164.4 | 203.4 | --- | --- | C2'endo | 0.0 |
| 1 | BA | A | 627 | --- | --- | 43.9 | 157.0 | --- | --- | C2'endo | 0.0 |
| 1 | BA | G | 1325 | --- | --- | 338.6 | 246.9 | --- | --- | C2'endo | 0.0 |
| 1 | BA | C | 2791 | --- | --- | 156.3 | 114.8 | 73.2 | 111.4 | C2'endo | 0.0 |
| 1 | CA | A | 8 | --- | --- | 297.5 | 245.0 | 312.5 | 262.7 | C2'endo | 0.248 |
| 1 | CA | A | 50 | --- | --- | 75.7 | 90.8 | 87.9 | 96.1 | C2'endo | 0.117 |
| 1 | CA | U | 189 | --- | --- | 323.5 | 245.0 | 313.6 | 216.7 | C2'endo | 0.034 |
| 1 | CA | A | 445 | --- | --- | 80.3 | 104.7 | --- | --- | C2'endo | 0.276 |
| 1 | CA | A | 454 | --- | --- | 69.8 | 195.2 | 36.9 | 149.3 | C2'endo | 0.0 |
| 1 | CA | A | 516 | --- | --- | 327.0 | 237.4 | 337.3 | 200.2 | C2'endo | 0.0 |
| 1 | CA | U | 1177 | --- | --- | 301.0 | 27.0 | --- | --- | C2'endo | 0.0 |
| 1 | CA | A | 1182 | --- | --- | 49.0 | 80.6 | 91.9 | 94.0 | C2'endo | 0.0 |
| 1 | CA | A | 1345 | --- | --- | 76.7 | 63.6 | --- | --- | C2'endo | 0.01 |
| 1 | DA | A | 196 | --- | --- | 327.7 | 245.1 | --- | --- | C2'endo | 0.0 |
| 1 | DA | A | 265 | --- | --- | 79.8 | 153.3 | --- | --- | C2'endo | 0.05 |
| 1 | DA | U | 271N | --- | --- | 12.5 | 354.5 | 344.7 | 34.2 | C2'endo | 0.0 |
| 1 | DA | A | 330 | --- | --- | 149.4 | 199.2 | --- | --- | C2'endo | 0.0 |
| 1 | DA | A | 457 | --- | --- | 8.8 | 150.5 | 18.2 | 154.0 | C2'endo | 0.0 |
| 1 | DA | G | 530 | --- | --- | 164.4 | 203.4 | --- | --- | C2'endo | 0.0 |
| 1 | DA | A | 627 | --- | --- | 43.9 | 156.9 | --- | --- | C2'endo | 0.0 |
| 1 | DA | G | 1325 | --- | --- | 338.6 | 246.9 | --- | --- | C2'endo | 0.0 |
| 1 | DA | C | 2791 | --- | --- | 156.3 | 114.7 | 73.2 | 111.4 | C2'endo | 0.0 |
| 1 | AA | U | 65 | --- | --- | --- | --- | 10.5 | 2.4 | C2'endo | 0.232 |
| 1 | AA | G | 123 | --- | --- | --- | --- | 287.6 | 17.0 | C2'endo | 0.06 |
| 1 | AA | A | 516 | --- | --- | --- | --- | 348.7 | 195.9 | C2'endo | 0.0 |
| 1 | AA | U | 544 | --- | --- | --- | --- | 61.3 | 111.1 | C2'endo | 0.0 |
| 1 | AA | A | 1139 | --- | --- | --- | --- | 73.0 | 76.7 | C2'endo | 0.0 |
| 1 | AA | U | 1221 | --- | --- | --- | --- | 30.0 | 10.2 | C2'endo | 0.514 |
| 1 | AW | C | 17 | --- | --- | --- | --- | 14.3 | 165.2 | C2'endo | 0.0 |
| 1 | AW | U | 47 | --- | --- | --- | --- | 22.1 | 213.1 | C2'endo | 0.0 |
| 1 | AV | U | 18 | --- | --- | --- | --- | 91.1 | 117.6 | C2'endo | 0.0 |
| 1 | BA | G | 321 | --- | --- | --- | --- | 337.4 | 297.9 | C2'endo | 0.51 |
| 1 | BA | G | 323 | 91.1 | 51.4 | --- | --- | 159.5 | 23.9 | C2'endo | 0.344 |
| 1 | BA | C | 456 | --- | --- | --- | --- | 46.9 | 91.5 | C2'endo | 0.0 |
| 1 | BA | C | 995 | --- | --- | --- | --- | 342.7 | 70.9 | C2'endo | 0.408 |
| 1 | BA | U | 1205 | --- | --- | --- | --- | 341.6 | 20.5 | C2'endo | 0.0 |
| 1 | BA | A | 1460 | --- | --- | --- | --- | 337.5 | 66.0 | C2'endo | 0.0 |
| 1 | BA | C | 1493 | --- | --- | --- | --- | 321.8 | 104.4 | C2'endo | 0.0 |
| 1 | BA | A | 1558 | --- | --- | --- | --- | 99.6 | 43.6 | C2'endo | 0.0 |
| 1 | BA | G | 1674 | --- | --- | --- | --- | 332.9 | 94.4 | C2'endo | 0.0 |
| 1 | BA | A | 1698 | --- | --- | --- | --- | 96.4 | 85.0 | C2'endo | 0.0 |
| 1 | BA | U | 1739 | --- | --- | --- | --- | 43.1 | 194.4 | C2'endo | 0.0 |
| 1 | BA | A | 1780 | --- | --- | --- | --- | 282.8 | 250.3 | C2'endo | 0.0 |
| 1 | BA | A | 1900 | --- | --- | --- | --- | 290.4 | 15.8 | C2'endo | 0.0 |
| 1 | BA | A | 2060 | --- | --- | --- | --- | 325.5 | 146.1 | C2'endo | 0.0 |
| 1 | BA | U | 2092 | --- | --- | --- | --- | 69.0 | 119.5 | C2'endo | 0.74 |
| 1 | BA | U | 2118 | --- | --- | --- | --- | 28.7 | 207.9 | C2'endo | 0.0 |
| 1 | BA | A | 2119 | --- | --- | --- | --- | 356.2 | 186.0 | C2'endo | 0.0 |
| 1 | BA | U | 2249 | --- | --- | --- | --- | 297.6 | 252.8 | C2'endo | 0.787 |
| 1 | BA | C | 2275 | --- | --- | --- | --- | 338.6 | 59.0 | C2'endo | 0.0 |
| 1 | BA | G | 2345 | --- | --- | --- | --- | 41.3 | 104.1 | C2'endo | 0.0 |
| 1 | BA | C | 2681 | --- | --- | --- | --- | 320.5 | 123.1 | C2'endo | 0.696 |
| 1 | BA | U | 2702 | --- | --- | --- | --- | 56.5 | 134.8 | C2'endo | 0.0 |
| 1 | BA | U | 2712 | 342.8 | 209.2 | --- | --- | 99.1 | 110.7 | C2'endo | 0.0 |
| 1 | BA | U | 2756 | 107.0 | 168.8 | --- | --- | 84.8 | 79.5 | C2'endo | 0.583 |
| 1 | BA | U | 2866 | --- | --- | --- | --- | 63.8 | 147.7 | C2'endo | 0.677 |
| 1 | CA | U | 65 | --- | --- | --- | --- | 356.0 | 85.4 | C2'endo | 0.34 |
| 1 | CA | G | 123 | --- | --- | --- | --- | 271.8 | 19.6 | C2'endo | 0.05 |
| 1 | CA | U | 544 | --- | --- | --- | --- | 75.1 | 118.6 | C2'endo | 0.0 |
| 1 | CA | G | 801 | --- | --- | --- | --- | 26.5 | 238.2 | C2'endo | 0.0 |
| 1 | CA | U | 937 | --- | --- | --- | --- | 284.4 | 267.0 | C2'endo | 0.0 |
| 1 | CA | A | 1139 | --- | --- | --- | --- | 71.4 | 77.4 | C2'endo | 0.0 |
| 1 | CA | G | 1163 | --- | --- | --- | --- | 245.8 | 10.2 | C2'endo | 0.0 |
| 1 | CA | U | 1221 | --- | --- | --- | --- | 40.3 | 14.0 | C2'endo | 0.385 |
| 1 | CW | U | 47 | --- | --- | --- | --- | 2.2 | 204.6 | C2'endo | 0.0 |
| 1 | DA | G | 321 | --- | --- | --- | --- | 337.4 | 297.9 | C2'endo | 0.51 |
| 1 | DA | G | 323 | 91.1 | 51.4 | --- | --- | 159.5 | 23.9 | C2'endo | 0.347 |
| 1 | DA | C | 456 | --- | --- | --- | --- | 46.9 | 91.4 | C2'endo | 0.0 |
| 1 | DA | C | 995 | --- | --- | --- | --- | 342.7 | 70.9 | C2'endo | 0.407 |
| 1 | DA | U | 1205 | --- | --- | --- | --- | 341.6 | 20.5 | C2'endo | 0.0 |
| 1 | DA | A | 1460 | --- | --- | --- | --- | 337.5 | 66.1 | C2'endo | 0.0 |
| 1 | DA | C | 1493 | --- | --- | --- | --- | 321.7 | 104.4 | C2'endo | 0.0 |
| 1 | DA | A | 1558 | --- | --- | --- | --- | 99.6 | 43.6 | C2'endo | 0.0 |
| 1 | DA | G | 1674 | --- | --- | --- | --- | 332.9 | 94.4 | C2'endo | 0.0 |
| 1 | DA | A | 1698 | --- | --- | --- | --- | 96.4 | 85.0 | C2'endo | 0.0 |
| 1 | DA | U | 1739 | --- | --- | --- | --- | 43.1 | 194.4 | C2'endo | 0.0 |
| 1 | DA | A | 1780 | --- | --- | --- | --- | 282.8 | 250.3 | C2'endo | 0.0 |
| 1 | DA | A | 1900 | --- | --- | --- | --- | 290.4 | 15.8 | C2'endo | 0.0 |
| 1 | DA | A | 2060 | --- | --- | --- | --- | 325.5 | 146.1 | C2'endo | 0.0 |
| 1 | DA | U | 2092 | --- | --- | --- | --- | 69.0 | 119.5 | C2'endo | 0.738 |
| 1 | DA | U | 2118 | --- | --- | --- | --- | 28.7 | 207.9 | C2'endo | 0.0 |
| 1 | DA | A | 2119 | --- | --- | --- | --- | 356.2 | 186.0 | C2'endo | 0.0 |
| 1 | DA | U | 2249 | --- | --- | --- | --- | 297.6 | 252.8 | C2'endo | 0.787 |
| 1 | DA | C | 2275 | --- | --- | --- | --- | 338.6 | 59.0 | C2'endo | 0.0 |
| 1 | DA | G | 2345 | --- | --- | --- | --- | 41.3 | 104.2 | C2'endo | 0.0 |
| 1 | DA | C | 2681 | --- | --- | --- | --- | 320.5 | 123.1 | C2'endo | 0.695 |
| 1 | DA | U | 2702 | --- | --- | --- | --- | 56.5 | 134.8 | C2'endo | 0.0 |
| 1 | DA | U | 2712 | 342.7 | 209.2 | --- | --- | 99.1 | 110.7 | C2'endo | 0.0 |
| 1 | DA | U | 2756 | 107.1 | 168.7 | --- | --- | 84.8 | 79.5 | C2'endo | 0.581 |
| 1 | DA | U | 2866 | --- | --- | --- | --- | 63.8 | 147.7 | C2'endo | 0.677 |