EMDataResource Pseudotorsion Plot Beta Server
RNA adopts complex three-dimensional folding patterns and has key roles in many cellular functions. Many cryoEM-derived structures contain RNA, including ribosomal particles, spliceosomes, RNA viruses, and polymerase complexes, yet procedures for validation of RNA structures are not as well developed as those for proteins.
This prototype web-service for virtual torsion angle validation of RNA structures can be applied to structures determined using cryo-EM as well as other structural methods. Virtual torsion angle plots for nucleic acids are analogous to Ramachandran plots for proteins. The tool evaluates single structures, either by file upload (mmCIF or PDB format) or by entering a PDB id.
The research community has developed simplified ways to represent nucleic acid backbone conformation through use of virtual torsion angles1-3. A common scheme is to define two virtual torsion angles (pseudotorsions), eta (η) and theta (θ) for a nucleotide triplet. The measured (η,θ) pair defines the backbone conformation relative to central residue (i).
The server calculates three varieties of pseudotorsion angles, as shown in the image below. In all definitions, phosphate atom positions are used, but the alternate atom differs: it is either the C4' atom (η,θ), C1' atom (η',θ'), or base origin4,5 position (η",θ").
Virtual torsion angles of the RNA backbone (calculated using DSSR4) are compared against the statistical distribution of a benchmark set of high quality, non-redundant6 RNA structures determined by X-ray crystallography at ≤ 2.5 Å. Separate analyses are performed based on sugar-puckering type (of ~27,000 residues in the benchmark set, ~24,000 are C3'-endo-like, ~3,000 are C2'-endo-like). Output plots and and a downloadable (csv format) table identify probable conformational outliers for any given RNA-containing structure. For comparison, RNA Suiteness7,8 scores are also provided in the csv (for residue i, suiteness of the i-1, i dinucleotide is listed. Suiteness range is 0(poor)-1(excellent). Calculation performed using DSSR).
The 2D plots permit comparison of pseudotorsion pairs for the structure being evaluated (plotted points) against population statistics of the benchmark set (smoothed contours: bar at right indicates benchmark residue count range). Outliers are marked on the plots by red crosses with residue information available on mouse hover. Summary statistics are provided below each plot to indicate the number of residues evaluated and percent outliers. Note that pseudotorsion angles cannot be calculated for terminal residues or around breaks in the polymer chain, so these are excluded from evaluation.
This tool is designed to identify regions of unusual conformation in folded RNA. Outliers can be legitimate but less-common backbone conformations, or they may indicate modelling error.
It may take several minutes to complete calculations for large structures such as ribosomes.
Please direct any questions/suggestions to help@emdataresource.org
- Olson, W. K. Configurational statistics of polynucleotide chains. An updated virtual bond model to treat effects of base stacking. Macromolecules 13, 721-728 (1980).
- Wadley, L. M., Keating, K. S., Duarte, C. M. & Pyle, A. M. Evaluating and learning from RNA pseudotorsional space: quantitative validation of a reduced representation for RNA structure. J Mol Biol 372, 942-957, doi (2007).
- Keating, K. S., Humphris, E. L. & Pyle, A. M. A new way to see RNA. Q Rev Biophys 44, 433-466, doi (2011).
- Lu, X. J., Bussemaker, H. J. & Olson, W. K. DSSR: an integrated software tool for dissecting the spatial structure of RNA. Nucleic Acids Res 43, e142, doi (2015).
- Li, S., Olson, W. K., Lu, X. J. Web 3DNA 2.0 for the analysis, visualization, and modeling of 3D nucleic acid structures. Nucleic Acids Research 47, W26-W34 (2019). doi
- Leontis, N. B., & Zirbel, C. L. Nonredundant 3D Structure Datasets for RNA Knowledge Extraction and Benchmarking. In RNA 3D Structure Analysis and Prediction, N. Leontis & E. Westhof (Eds.), (Vol. 27, pp. 281-298). Springer Berlin Heidelberg. doi (2012).
- Richardson, J. S., Schneider, B., Murray, L. W., Kapral, G. J., Immormino, R.M., Headd, J. J., Richardson, D. C., Ham, D., Hershkovits, E., Williams, L. D., Keating, K. S., Pyle, A. M., Micallef, D., Westbrook, J., Berman, H. M. RNA backbone: consensus all-angle conformers and modular string nomenclature (an RNA Ontology Consortium contribution). RNA 14, 465-81, doi (2008).
- Chen, V. B., Arendall III, W. B., Headd, J. J., Keedy, D. A., Immormino, R. M., Kapral, G. J., Murray, L. W., Richardson, J. S., Richardson, D. C., MolProbity: all-atom structure validation for macromolecular crystallography, Acta Cryst D 66, 12-21, doi (2010)
RNA Pseudotorsion Plots for PDB entry 5el7
Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with a U-U mismatch in the second position and antibiotic paromomycin Deposited 2015-11-04 X-RAY DIFFRACTION resol 3.15 Å
8098 C3'-endo RNA residues with 189 outliers ( 2.33 % ) from Eta-Theta plot
1177 C2'-endo RNA residues with 137 outliers ( 11.64 % ) from Eta-Theta plot
8098 C3'-endo RNA residues with 166 outliers ( 2.05 % ) from Eta'-Theta' plot
1177 C2'-endo RNA residues with 93 outliers ( 7.9 % ) from Eta'-Theta' plot
8098 C3'-endo RNA residues with 138 outliers ( 1.7 % ) from Eta"-Theta" plot
1177 C2'-endo RNA residues with 67 outliers ( 5.69 % ) from Eta"-Theta" plot
List of Outliers
| Model Number | Chain | Residue Type | Residue Identifier | Eta | Theta | Eta' | Theta' | Eta'' | Theta'' | Sugar Pucker | Suiteness |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 13 | A | 160 | 346.0 | 280.7 | 27.5 | 278.5 | 19.4 | 324.0 | C3'endo | 0.0 |
| 1 | 13 | U | 208 | 82.3 | 309.9 | 88.2 | 287.5 | --- | --- | C3'endo | 0.0 |
| 1 | 13 | U | 210 | 244.6 | 37.4 | 218.4 | 46.6 | --- | --- | C3'endo | 0.442 |
| 1 | 13 | G | 346 | 86.2 | 282.2 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | 13 | C | 352 | 121.4 | 87.0 | 107.1 | 71.6 | 110.9 | 356.7 | C3'endo | 0.0 |
| 1 | 13 | A | 353 | 4.6 | 129.2 | 359.4 | 126.1 | --- | --- | C3'endo | 0.0 |
| 1 | 13 | C | 466 | 42.5 | 285.3 | 54.2 | 295.8 | --- | --- | C3'endo | 0.0 |
| 1 | 13 | A | 563 | 107.4 | 108.8 | 103.1 | 85.4 | 98.0 | 30.8 | C3'endo | 0.369 |
| 1 | 13 | U | 723 | 66.5 | 296.0 | 72.6 | 292.4 | --- | --- | C3'endo | 0.0 |
| 1 | 13 | C | 754 | 119.6 | 299.7 | 103.5 | 307.7 | --- | --- | C3'endo | 0.847 |
| 1 | 13 | A | 816 | 71.3 | 30.9 | --- | --- | --- | --- | C3'endo | 0.553 |
| 1 | 13 | U | 841 | 66.7 | 0.4 | 74.5 | 323.0 | --- | --- | C3'endo | 0.0 |
| 1 | 13 | U | 843 | 0.4 | 315.3 | 42.3 | 280.3 | --- | --- | C3'endo | 0.0 |
| 1 | 13 | C | 1028 | 78.2 | 277.3 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | 13 | G | 1032A | 267.1 | 127.5 | 293.0 | 155.1 | 323.6 | 138.5 | C3'endo | 0.0 |
| 1 | 13 | U | 1125 | 346.6 | 81.7 | 22.9 | 10.2 | --- | --- | C3'endo | 0.0 |
| 1 | 13 | U | 1136 | 87.8 | 0.6 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | 13 | A | 1183 | 19.9 | 304.2 | 342.4 | 283.2 | 351.8 | 195.0 | C3'endo | 0.0 |
| 1 | 13 | U | 1240 | 15.1 | 336.4 | 20.6 | 331.7 | 68.3 | 351.8 | C3'endo | 0.0 |
| 1 | 13 | U | 1257 | 334.6 | 352.7 | 355.6 | 327.2 | 186.0 | 342.4 | C3'endo | 0.0 |
| 1 | 13 | U | 1278 | 238.2 | 42.2 | 240.5 | 47.0 | --- | --- | C3'endo | 0.0 |
| 1 | 13 | A | 1451 | 65.3 | 16.1 | 85.1 | 340.0 | --- | --- | C3'endo | 0.0 |
| 1 | 13 | G | 1453 | 3.5 | 305.8 | 310.7 | 340.3 | --- | --- | C3'endo | 0.0 |
| 1 | 1K | A | 59 | 52.2 | 260.6 | --- | --- | --- | --- | C3'endo | 0.129 |
| 1 | 2K | A | 22 | 242.6 | 135.5 | 262.1 | 147.8 | --- | --- | C3'endo | 0.0 |
| 1 | 3K | U | 16 | 279.1 | 124.3 | 291.2 | 152.6 | --- | --- | C3'endo | 0.0 |
| 1 | 3K | G | 19 | 224.5 | 279.2 | 225.3 | 275.8 | --- | --- | C3'endo | 0.0 |
| 1 | 3K | U | 44 | 208.0 | 3.1 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | 3K | G | 46 | 35.3 | 149.8 | 35.7 | 130.6 | 51.8 | 357.5 | C3'endo | 0.0 |
| 1 | 3K | U | 47 | 28.0 | 3.5 | 20.6 | 356.9 | 137.8 | 9.1 | C3'endo | 0.0 |
| 1 | 3K | G | 49 | 352.1 | 167.9 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | 1H | U | 162 | 35.8 | 36.6 | 57.0 | 34.5 | 43.3 | 183.1 | C3'endo | 0.12 |
| 1 | 1H | U | 163 | 222.2 | 329.3 | 223.9 | 320.3 | --- | --- | C3'endo | 0.0 |
| 1 | 1H | G | 248 | 119.9 | 304.3 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | 1H | U | 270L | 8.1 | 22.8 | 44.1 | 309.9 | --- | --- | C3'endo | 0.01 |
| 1 | 1H | U | 270O | 51.9 | 298.8 | 57.3 | 280.6 | --- | --- | C3'endo | 0.0 |
| 1 | 1H | U | 271C | 61.0 | 297.8 | 52.3 | 297.2 | --- | --- | C3'endo | 0.0 |
| 1 | 1H | G | 275 | 253.0 | 105.6 | 239.8 | 120.1 | --- | --- | C3'endo | 0.0 |
| 1 | 1H | A | 443 | 270.5 | 84.2 | 312.4 | 65.0 | 16.3 | 15.2 | C3'endo | 0.0 |
| 1 | 1H | A | 480 | 112.0 | 304.8 | --- | --- | --- | --- | C3'endo | 0.041 |
| 1 | 1H | U | 504 | 321.6 | 95.6 | 315.1 | 71.5 | 341.8 | 358.6 | C3'endo | 0.0 |
| 1 | 1H | A | 526 | 211.7 | 108.0 | --- | --- | --- | --- | C3'endo | 0.514 |
| 1 | 1H | A | 572 | 59.7 | 260.1 | --- | --- | --- | --- | C3'endo | 0.51 |
| 1 | 1H | G | 615 | 354.7 | 167.3 | 46.4 | 141.6 | 64.1 | 90.7 | C3'endo | 0.0 |
| 1 | 1H | C | 645 | 88.5 | 338.3 | 79.0 | 286.8 | --- | --- | C3'endo | 0.0 |
| 1 | 1H | U | 747 | 321.5 | 28.1 | 346.8 | 14.1 | 142.8 | 9.8 | C3'endo | 0.017 |
| 1 | 1H | A | 751 | 301.6 | 100.5 | 324.5 | 121.7 | 317.8 | 121.8 | C3'endo | 0.398 |
| 1 | 1H | G | 881 | 131.4 | 339.9 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | 1H | U | 958 | 315.6 | 85.2 | 6.5 | 64.4 | --- | --- | C3'endo | 0.0 |
| 1 | 1H | C | 1135 | 89.9 | 260.3 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | 1H | G | 1136 | 16.5 | 158.1 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | 1H | U | 1273 | 327.8 | 143.3 | 29.9 | 110.6 | 66.1 | 75.6 | C3'endo | 0.0 |
| 1 | 1H | U | 1420 | 69.4 | 261.7 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | 1H | A | 1510 | 260.5 | 105.0 | 283.6 | 107.4 | --- | --- | C3'endo | 0.0 |
| 1 | 1H | G | 1534 | 83.4 | 126.9 | 96.6 | 137.5 | 50.3 | 104.7 | C3'endo | 0.0 |
| 1 | 1H | A | 1536 | 284.9 | 305.5 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | 1H | U | 1730 | 31.2 | 290.2 | 77.4 | 272.1 | --- | --- | C3'endo | 0.0 |
| 1 | 1H | U | 1757 | 323.6 | 358.2 | 328.4 | 334.3 | 342.1 | 342.1 | C3'endo | 0.01 |
| 1 | 1H | C | 1965 | 270.4 | 335.0 | 345.1 | 293.2 | --- | --- | C3'endo | 0.0 |
| 1 | 1H | A | 1971 | 5.3 | 344.0 | 354.3 | 334.3 | --- | --- | C3'endo | 0.0 |
| 1 | 1H | C | 2055 | 233.0 | 50.8 | 253.9 | 57.9 | 305.0 | 128.2 | C3'endo | 0.0 |
| 1 | 1H | A | 2062 | 101.1 | 272.3 | --- | --- | --- | --- | C3'endo | 0.152 |
| 1 | 1H | G | 2112 | 81.7 | 104.2 | 75.6 | 119.2 | --- | --- | C3'endo | 0.0 |
| 1 | 1H | G | 2115 | 122.9 | 290.4 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | 1H | A | 2119 | 271.8 | 289.9 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | 1H | U | 2132 | 21.6 | 35.9 | 33.3 | 32.1 | 110.1 | 55.1 | C3'endo | 0.0 |
| 1 | 1H | G | 2133 | 217.2 | 110.0 | 233.7 | 124.0 | --- | --- | C3'endo | 0.0 |
| 1 | 1H | G | 2147 | 35.7 | 304.4 | 64.4 | 292.0 | --- | --- | C3'endo | 0.0 |
| 1 | 1H | G | 2157 | 222.2 | 133.1 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | 1H | A | 2212 | 132.4 | 95.6 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | 1H | C | 2424 | 94.7 | 333.0 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | 1H | G | 2428 | 289.8 | 104.5 | 273.2 | 99.2 | --- | --- | C3'endo | 0.014 |
| 1 | 1H | G | 2468 | 213.5 | 122.3 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | 1H | U | 2473 | 281.8 | 280.0 | --- | --- | --- | --- | C3'endo | 0.385 |
| 1 | 1H | G | 2505 | 344.4 | 143.2 | 346.1 | 138.3 | 331.7 | 111.6 | C3'endo | 0.0 |
| 1 | 1H | A | 2712A | 92.4 | 334.0 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | 1H | A | 2765 | 84.5 | 264.2 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | 1H | G | 2795 | 82.8 | 264.2 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | 1H | U | 2797 | 295.7 | 314.2 | 333.6 | 288.0 | 348.5 | 298.4 | C3'endo | 0.0 |
| 1 | 1H | G | 2833 | 358.9 | 4.8 | 26.3 | 342.8 | 102.4 | 343.8 | C3'endo | 0.0 |
| 1 | 16 | U | 41 | 289.6 | 338.4 | 331.4 | 311.4 | --- | --- | C3'endo | 0.213 |
| 1 | 16 | G | 44 | 278.5 | 133.5 | 306.6 | 88.7 | 296.1 | 71.0 | C3'endo | 0.542 |
| 1 | 1G | G | 247 | 258.9 | 132.3 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | 1G | C | 352 | 89.0 | 113.6 | 90.6 | 77.8 | 109.9 | 359.0 | C3'endo | 0.0 |
| 1 | 1G | A | 353 | 11.6 | 120.5 | 5.9 | 128.6 | --- | --- | C3'endo | 0.0 |
| 1 | 1G | C | 466 | 28.6 | 312.4 | 55.5 | 296.7 | --- | --- | C3'endo | 0.0 |
| 1 | 1G | A | 563 | 102.3 | 107.3 | 98.5 | 82.5 | 95.2 | 26.0 | C3'endo | 0.01 |
| 1 | 1G | U | 723 | 38.2 | 323.7 | 49.2 | 322.1 | 36.0 | 334.5 | C3'endo | 0.0 |
| 1 | 1G | C | 754 | 121.4 | 308.3 | 103.6 | 317.6 | --- | --- | C3'endo | 0.0 |
| 1 | 1G | A | 816 | 73.1 | 26.9 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | 1G | U | 841 | 346.6 | 117.0 | 21.3 | 54.0 | 30.7 | 357.1 | C3'endo | 0.0 |
| 1 | 1G | U | 843 | 82.4 | 268.1 | 64.1 | 301.7 | --- | --- | C3'endo | 0.0 |
| 1 | 1G | U | 1025 | 133.7 | 78.7 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | 1G | G | 1029 | 212.0 | 114.9 | 242.1 | 102.5 | 284.7 | 75.0 | C3'endo | 0.0 |
| 1 | 1G | G | 1094 | 19.5 | 45.1 | 8.1 | 51.3 | 357.5 | 48.9 | C3'endo | 0.0 |
| 1 | 1G | U | 1125 | 340.8 | 139.1 | 342.3 | 149.0 | 358.9 | 130.7 | C3'endo | 0.0 |
| 1 | 1G | U | 1136 | 34.2 | 27.4 | 63.6 | 331.2 | --- | --- | C3'endo | 0.019 |
| 1 | 1G | C | 1145 | 213.4 | 125.5 | --- | --- | --- | --- | C3'endo | 0.634 |
| 1 | 1G | A | 1183 | 351.5 | 316.1 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | 1G | C | 1200 | 214.0 | 32.7 | 220.2 | 20.7 | --- | --- | C3'endo | 0.0 |
| 1 | 1G | U | 1257 | 330.1 | 2.2 | 345.4 | 333.4 | 151.5 | 343.3 | C3'endo | 0.0 |
| 1 | 1G | U | 1278 | 232.2 | 44.7 | 226.9 | 49.6 | --- | --- | C3'endo | 0.019 |
| 1 | 1G | G | 1300 | 290.2 | 56.4 | 283.5 | 70.9 | --- | --- | C3'endo | 0.0 |
| 1 | 1G | C | 1397 | 346.0 | 38.5 | 28.0 | 5.1 | 93.8 | 347.0 | C3'endo | 0.0 |
| 1 | 1G | C | 1449 | 205.1 | 298.2 | --- | --- | --- | --- | C3'endo | 0.06 |
| 1 | 1G | U | 1450 | 235.7 | 337.5 | 258.3 | 338.7 | 315.0 | 334.4 | C3'endo | 0.0 |
| 1 | 1G | A | 1451 | 97.4 | 26.8 | 141.0 | 343.0 | --- | --- | C3'endo | 0.0 |
| 1 | 1G | G | 1453 | 28.8 | 300.1 | 332.1 | 349.3 | 26.7 | 336.6 | C3'endo | 0.0 |
| 1 | 1G | U | 1532 | 253.9 | 271.6 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | 2L | A | 22 | 243.8 | 130.9 | 254.1 | 145.5 | --- | --- | C3'endo | 0.0 |
| 1 | 3L | G | 19 | 278.7 | 309.6 | 253.0 | 306.2 | --- | --- | C3'endo | 0.0 |
| 1 | 3L | U | 20 | 84.8 | 288.9 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | 3L | G | 45 | 116.9 | 8.0 | 145.5 | 336.3 | 151.9 | 329.3 | C3'endo | 0.0 |
| 1 | 3L | A | 59 | 37.5 | 285.0 | 56.3 | 282.8 | --- | --- | C3'endo | 0.0 |
| 1 | 14 | C | 4 | 265.3 | 297.1 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | 14 | U | 90 | 99.2 | 94.1 | 127.3 | 90.7 | --- | --- | C3'endo | 0.0 |
| 1 | 14 | C | 155 | 124.7 | 332.7 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | 14 | U | 162 | 211.5 | 117.7 | 243.6 | 83.3 | 285.9 | 71.9 | C3'endo | 0.0 |
| 1 | 14 | G | 248 | 116.7 | 302.1 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | 14 | U | 270O | 40.7 | 289.8 | 19.8 | 306.3 | 8.9 | 217.2 | C3'endo | 0.0 |
| 1 | 14 | C | 277 | 94.7 | 291.7 | 115.0 | 286.7 | --- | --- | C3'endo | 0.0 |
| 1 | 14 | U | 405 | 28.7 | 322.7 | 34.6 | 309.6 | --- | --- | C3'endo | 0.0 |
| 1 | 14 | A | 443 | 275.5 | 79.9 | 318.7 | 60.9 | 13.1 | 16.2 | C3'endo | 0.0 |
| 1 | 14 | C | 456 | 301.2 | 137.9 | 3.8 | 101.3 | 63.9 | 7.4 | C3'endo | 0.0 |
| 1 | 14 | A | 480 | 106.5 | 306.5 | 82.8 | 286.0 | --- | --- | C3'endo | 0.0 |
| 1 | 14 | U | 504 | 327.4 | 87.7 | 323.9 | 64.1 | 357.4 | 343.6 | C3'endo | 0.0 |
| 1 | 14 | C | 645 | 68.5 | 332.2 | 77.3 | 298.0 | --- | --- | C3'endo | 0.0 |
| 1 | 14 | U | 747 | 327.5 | 26.8 | 3.8 | 13.2 | 144.0 | 14.4 | C3'endo | 0.185 |
| 1 | 14 | A | 751 | 296.8 | 105.9 | 323.9 | 122.8 | 321.3 | 120.1 | C3'endo | 0.351 |
| 1 | 14 | A | 781 | 335.0 | 117.3 | 348.4 | 87.1 | 344.6 | 25.6 | C3'endo | 0.661 |
| 1 | 14 | A | 784 | 308.9 | 111.3 | 309.3 | 101.3 | --- | --- | C3'endo | 0.0 |
| 1 | 14 | U | 958 | 323.3 | 86.8 | 5.5 | 32.7 | --- | --- | C3'endo | 0.183 |
| 1 | 14 | A | 1070 | 123.8 | 61.1 | 119.1 | 43.8 | --- | --- | C3'endo | 0.0 |
| 1 | 14 | G | 1071 | 310.0 | 297.2 | 318.4 | 264.7 | --- | --- | C3'endo | 0.0 |
| 1 | 14 | C | 1072 | 124.3 | 60.8 | 148.6 | 51.8 | 142.8 | 13.3 | C3'endo | 0.0 |
| 1 | 14 | G | 1112 | 245.5 | 116.2 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | 14 | C | 1135 | 83.9 | 270.4 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | 14 | G | 1136 | 9.4 | 165.4 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | 14 | A | 1174 | 93.9 | 289.3 | 75.1 | 272.4 | --- | --- | C3'endo | 0.0 |
| 1 | 14 | U | 1175 | 268.3 | 109.3 | 304.1 | 127.6 | 306.6 | 142.6 | C3'endo | 0.0 |
| 1 | 14 | A | 1177 | 241.0 | 70.2 | 237.5 | 70.6 | --- | --- | C3'endo | 0.0 |
| 1 | 14 | U | 1273 | 332.3 | 143.6 | 32.6 | 114.0 | 65.5 | 72.2 | C3'endo | 0.01 |
| 1 | 14 | U | 1420 | 64.0 | 274.0 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | 14 | A | 1444A | 92.2 | 313.2 | 96.9 | 292.3 | --- | --- | C3'endo | 0.0 |
| 1 | 14 | C | 1509 | 282.3 | 321.2 | 255.0 | 338.5 | --- | --- | C3'endo | 0.0 |
| 1 | 14 | C | 1585 | 21.8 | 291.7 | 44.1 | 291.9 | --- | --- | C3'endo | 0.0 |
| 1 | 14 | U | 1757 | 330.7 | 347.7 | 334.7 | 326.7 | 22.4 | 323.5 | C3'endo | 0.365 |
| 1 | 14 | C | 1965 | 270.8 | 333.5 | 341.0 | 293.1 | --- | --- | C3'endo | 0.122 |
| 1 | 14 | A | 1969 | 216.1 | 89.5 | --- | --- | --- | --- | C3'endo | 0.286 |
| 1 | 14 | A | 1971 | 12.2 | 342.3 | 357.5 | 333.2 | --- | --- | C3'endo | 0.0 |
| 1 | 14 | A | 2030 | 10.8 | 107.4 | 39.1 | 102.9 | 19.1 | 80.0 | C3'endo | 0.7 |
| 1 | 14 | C | 2055 | 230.8 | 49.2 | 250.4 | 58.7 | 304.7 | 122.7 | C3'endo | 0.0 |
| 1 | 14 | A | 2062 | 102.4 | 274.3 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | 14 | A | 2119 | 265.7 | 283.4 | --- | --- | --- | --- | C3'endo | 0.043 |
| 1 | 14 | G | 2131 | 212.0 | 26.1 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | 14 | G | 2133 | 226.1 | 32.0 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | 14 | U | 2144 | 222.2 | 124.9 | 238.2 | 131.0 | --- | --- | C3'endo | 0.027 |
| 1 | 14 | G | 2153 | 114.5 | 136.6 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | 14 | U | 2213 | 55.2 | 286.9 | 63.8 | 285.8 | --- | --- | C3'endo | 0.085 |
| 1 | 14 | A | 2267 | 129.5 | 35.3 | 145.5 | 32.4 | --- | --- | C3'endo | 0.0 |
| 1 | 14 | C | 2347 | 132.1 | 137.9 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | 14 | C | 2424 | 92.8 | 340.0 | --- | --- | --- | --- | C3'endo | 0.05 |
| 1 | 14 | G | 2428 | 291.3 | 101.2 | 272.8 | 95.5 | --- | --- | C3'endo | 0.025 |
| 1 | 14 | U | 2473 | 269.6 | 281.4 | 319.6 | 271.9 | --- | --- | C3'endo | 0.166 |
| 1 | 14 | G | 2505 | 342.3 | 142.0 | 344.8 | 141.8 | 329.0 | 119.2 | C3'endo | 0.0 |
| 1 | 14 | G | 2529 | 39.8 | 64.9 | 38.3 | 27.2 | --- | --- | C3'endo | 0.0 |
| 1 | 14 | C | 2573 | 272.1 | 67.6 | 260.4 | 71.2 | --- | --- | C3'endo | 0.0 |
| 1 | 14 | G | 2578 | 46.8 | 125.7 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | 14 | G | 2630 | 11.5 | 169.6 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | 14 | A | 2712A | 99.9 | 331.1 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | 14 | A | 2750 | 30.3 | 277.7 | 94.2 | 147.8 | 71.0 | 81.5 | C3'endo | 0.0 |
| 1 | 14 | G | 2777 | 345.0 | 105.8 | 329.8 | 65.4 | --- | --- | C3'endo | 0.0 |
| 1 | 14 | C | 2791 | 45.9 | 71.1 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | 14 | U | 2797 | 275.1 | 309.7 | 300.9 | 312.1 | 313.5 | 318.7 | C3'endo | 0.0 |
| 1 | 14 | G | 2833 | 18.1 | 341.6 | 50.8 | 316.9 | --- | --- | C3'endo | 0.0 |
| 1 | 14 | G | 2893 | 217.3 | 29.5 | 230.0 | 49.8 | --- | --- | C3'endo | 0.0 |
| 1 | 14 | G | 2894 | 217.4 | 47.4 | --- | --- | --- | --- | C3'endo | 0.0 |
| 1 | 1J | U | 41 | 305.6 | 328.6 | 343.0 | 304.8 | --- | --- | C3'endo | 0.613 |
| 1 | 1J | G | 44 | 296.8 | 131.2 | 308.8 | 94.1 | 298.2 | 71.3 | C3'endo | 0.477 |
| 1 | 13 | A | 722 | --- | --- | 116.6 | 139.3 | 152.3 | 59.2 | C3'endo | 0.818 |
| 1 | 13 | C | 1362A | --- | --- | 131.4 | 9.6 | --- | --- | C3'endo | 0.01 |
| 1 | 13 | A | 1398 | --- | --- | 153.9 | 83.0 | --- | --- | C3'endo | 0.0 |
| 1 | 3K | A | 21 | --- | --- | 269.6 | 169.6 | --- | --- | C3'endo | 0.0 |
| 1 | 1H | A | 195 | --- | --- | 213.4 | 329.9 | --- | --- | C3'endo | 0.0 |
| 1 | 1H | G | 270N | --- | --- | 113.7 | 116.2 | 87.9 | 52.2 | C3'endo | 0.0 |
| 1 | 1H | G | 763 | --- | --- | 125.0 | 140.3 | 102.5 | 112.6 | C3'endo | 0.526 |
| 1 | 1H | A | 781 | --- | --- | 348.0 | 82.2 | 342.6 | 25.7 | C3'endo | 0.418 |
| 1 | 1H | A | 1785 | --- | --- | 117.3 | 144.7 | 123.9 | 93.4 | C3'endo | 0.641 |
| 1 | 1H | G | 2131 | --- | --- | 145.2 | 53.6 | --- | --- | C3'endo | 0.0 |
| 1 | 1H | C | 2145 | --- | --- | 104.8 | 290.7 | --- | --- | C3'endo | 0.0 |
| 1 | 1H | C | 2146 | --- | --- | 244.2 | 108.9 | 273.4 | 48.6 | C3'endo | 0.016 |
| 1 | 1H | C | 2573 | --- | --- | 259.1 | 54.0 | --- | --- | C3'endo | 0.0 |
| 1 | 1H | A | 2577 | --- | --- | 279.9 | 353.4 | --- | --- | C3'endo | 0.11 |
| 1 | 1H | C | 2755 | --- | --- | 349.6 | 150.8 | 349.5 | 102.0 | C3'endo | 0.0 |
| 1 | 16 | A | 13 | --- | --- | 256.3 | 154.0 | --- | --- | C3'endo | 0.0 |
| 1 | 1G | C | 842 | --- | --- | 132.3 | 60.3 | --- | --- | C3'endo | 0.0 |
| 1 | 1G | A | 1004 | --- | --- | 8.6 | 165.3 | 2.3 | 114.2 | C3'endo | 0.0 |
| 1 | 1G | A | 1005 | --- | --- | 132.1 | 294.7 | --- | --- | C3'endo | 0.168 |
| 1 | 1G | C | 1028B | --- | --- | 149.2 | 287.8 | --- | --- | C3'endo | 0.028 |
| 1 | 1G | A | 1032 | --- | --- | 131.3 | 282.6 | --- | --- | C3'endo | 0.0 |
| 1 | 1G | G | 1182 | --- | --- | 232.4 | 55.5 | 301.5 | 339.0 | C3'endo | 0.0 |
| 1 | 1G | U | 1212 | --- | --- | 242.9 | 45.6 | --- | --- | C3'endo | 0.0 |
| 1 | 1G | A | 1225 | --- | --- | 237.0 | 270.3 | --- | --- | C3'endo | 0.0 |
| 1 | 1G | C | 1362A | --- | --- | 128.4 | 359.9 | --- | --- | C3'endo | 0.0 |
| 1 | 1L | G | 49 | --- | --- | 272.0 | 174.0 | --- | --- | C3'endo | 0.0 |
| 1 | 14 | U | 161 | --- | --- | 43.5 | 340.2 | --- | --- | C3'endo | 0.0 |
| 1 | 14 | A | 195 | --- | --- | 212.8 | 326.3 | --- | --- | C3'endo | 0.451 |
| 1 | 14 | A | 412 | --- | --- | 264.2 | 263.5 | --- | --- | C3'endo | 0.0 |
| 1 | 14 | G | 615 | --- | --- | 283.9 | 163.1 | 291.8 | 115.1 | C3'endo | 0.0 |
| 1 | 14 | G | 763 | --- | --- | 124.4 | 141.0 | 111.3 | 113.6 | C3'endo | 0.276 |
| 1 | 14 | G | 1056 | --- | --- | 212.6 | 44.8 | 301.4 | 46.0 | C3'endo | 0.0 |
| 1 | 14 | A | 1086 | --- | --- | 142.7 | 81.3 | 164.0 | 61.2 | C3'endo | 0.0 |
| 1 | 14 | G | 1087 | --- | --- | 87.4 | 148.5 | 118.3 | 121.5 | C3'endo | 0.0 |
| 1 | 14 | G | 1416 | --- | --- | 307.5 | 134.3 | --- | --- | C3'endo | 0.0 |
| 1 | 14 | A | 1543 | --- | --- | 119.4 | 135.5 | 115.8 | 73.0 | C3'endo | 0.0 |
| 1 | 14 | U | 1779 | --- | --- | 151.7 | 32.5 | 180.3 | 347.9 | C3'endo | 0.01 |
| 1 | 14 | A | 1785 | --- | --- | 113.3 | 145.1 | 125.2 | 93.3 | C3'endo | 0.559 |
| 1 | 14 | A | 2320 | --- | --- | 146.0 | 87.3 | --- | --- | C3'endo | 0.04 |
| 1 | 14 | A | 2476 | --- | --- | 320.4 | 275.7 | 349.3 | 311.3 | C3'endo | 0.0 |
| 1 | 14 | A | 2577 | --- | --- | 275.9 | 359.4 | --- | --- | C3'endo | 0.223 |
| 1 | 14 | C | 2755 | --- | --- | 352.4 | 149.1 | 6.3 | 86.4 | C3'endo | 0.235 |
| 1 | 13 | G | 6 | --- | --- | --- | --- | 113.0 | 105.4 | C3'endo | 0.0 |
| 1 | 13 | C | 345 | --- | --- | --- | --- | 201.6 | 47.3 | C3'endo | 0.0 |
| 1 | 13 | U | 560 | 6.5 | 84.0 | --- | --- | 58.1 | 340.4 | C3'endo | 0.464 |
| 1 | 13 | A | 665 | --- | --- | --- | --- | 14.4 | 158.5 | C3'endo | 0.0 |
| 1 | 13 | A | 1377 | --- | --- | --- | --- | 172.2 | 77.6 | C3'endo | 0.343 |
| 1 | 3K | U | 17 | --- | --- | --- | --- | 359.3 | 184.4 | C3'endo | 0.0 |
| 1 | 3K | C | 65 | --- | --- | --- | --- | 332.1 | 154.8 | C3'endo | 0.0 |
| 1 | 4K | A | 13 | --- | --- | --- | --- | 340.2 | 175.5 | C3'endo | 0.0 |
| 1 | 1H | G | 271 | --- | --- | --- | --- | 354.7 | 191.1 | C3'endo | 0.0 |
| 1 | 1H | G | 274 | --- | --- | --- | --- | 22.9 | 180.5 | C3'endo | 0.0 |
| 1 | 1H | A | 276 | --- | --- | --- | --- | 76.8 | 157.3 | C3'endo | 0.293 |
| 1 | 1H | C | 277 | --- | --- | --- | --- | 165.2 | 336.1 | C3'endo | 0.0 |
| 1 | 1H | G | 388 | --- | --- | --- | --- | 71.7 | 137.3 | C3'endo | 0.781 |
| 1 | 1H | U | 613 | --- | --- | --- | --- | 286.7 | 58.2 | C3'endo | 0.0 |
| 1 | 1H | A | 654 | --- | --- | --- | --- | 18.4 | 115.1 | C3'endo | 0.0 |
| 1 | 1H | A | 1027 | --- | --- | --- | --- | 32.2 | 165.8 | C3'endo | 0.0 |
| 1 | 1H | A | 1127 | --- | --- | --- | --- | 97.8 | 137.0 | C3'endo | 0.0 |
| 1 | 1H | U | 1142 | 321.7 | 20.3 | --- | --- | 268.2 | 16.4 | C3'endo | 0.0 |
| 1 | 1H | A | 1274 | --- | --- | --- | --- | 6.4 | 132.7 | C3'endo | 0.0 |
| 1 | 1H | A | 1384 | --- | --- | --- | --- | 105.1 | 165.8 | C3'endo | 0.172 |
| 1 | 1H | C | 1546 | --- | --- | --- | --- | 270.2 | 63.4 | C3'endo | 0.458 |
| 1 | 1H | C | 1646 | --- | --- | --- | --- | 193.6 | 24.3 | C3'endo | 0.0 |
| 1 | 1H | A | 1700 | --- | --- | --- | --- | 345.2 | 299.3 | C3'endo | 0.01 |
| 1 | 1H | U | 1779 | --- | --- | --- | --- | 185.2 | 342.2 | C3'endo | 0.242 |
| 1 | 1H | G | 1816 | 315.0 | 10.7 | --- | --- | 292.1 | 82.2 | C3'endo | 0.0 |
| 1 | 1H | G | 2116 | --- | --- | --- | --- | 55.4 | 148.6 | C3'endo | 0.0 |
| 1 | 1H | U | 2167 | --- | --- | --- | --- | 48.3 | 177.0 | C3'endo | 0.732 |
| 1 | 1H | G | 2168 | --- | --- | --- | --- | 133.7 | 73.9 | C3'endo | 0.0 |
| 1 | 1H | A | 2530 | --- | --- | --- | --- | 116.7 | 136.9 | C3'endo | 0.485 |
| 1 | 1H | U | 2688 | --- | --- | --- | --- | 282.1 | 333.4 | C3'endo | 0.075 |
| 1 | 1H | G | 2872 | --- | --- | --- | --- | 80.6 | 128.7 | C3'endo | 0.0 |
| 1 | 1H | A | 2883 | --- | --- | --- | --- | 161.6 | 331.5 | C3'endo | 0.175 |
| 1 | 1G | A | 397 | --- | --- | --- | --- | 325.4 | 65.3 | C3'endo | 0.0 |
| 1 | 1G | U | 560 | 358.5 | 88.1 | --- | --- | 56.3 | 350.3 | C3'endo | 0.462 |
| 1 | 1G | A | 665 | --- | --- | --- | --- | 14.3 | 144.6 | C3'endo | 0.0 |
| 1 | 1G | A | 977 | --- | --- | --- | --- | 72.7 | 182.3 | C3'endo | 0.0 |
| 1 | 1G | C | 1030 | --- | --- | --- | --- | 47.2 | 150.5 | C3'endo | 0.0 |
| 1 | 1G | G | 1202 | --- | --- | --- | --- | 305.3 | 297.7 | C3'endo | 0.0 |
| 1 | 3L | U | 17 | 63.3 | 269.3 | --- | --- | 327.2 | 158.8 | C3'endo | 0.0 |
| 1 | 3L | U | 34 | --- | --- | --- | --- | 35.6 | 184.7 | C3'endo | 0.0 |
| 1 | 14 | A | 5 | --- | --- | --- | --- | 294.8 | 135.6 | C3'endo | 0.0 |
| 1 | 14 | G | 271 | --- | --- | --- | --- | 322.9 | 192.9 | C3'endo | 0.0 |
| 1 | 14 | G | 351 | --- | --- | --- | --- | 259.8 | 34.4 | C3'endo | 0.752 |
| 1 | 14 | G | 388 | --- | --- | --- | --- | 69.6 | 143.4 | C3'endo | 0.394 |
| 1 | 14 | A | 654 | --- | --- | --- | --- | 92.3 | 123.1 | C3'endo | 0.0 |
| 1 | 14 | G | 879 | --- | --- | --- | --- | 318.9 | 158.1 | C3'endo | 0.0 |
| 1 | 14 | G | 1055 | --- | --- | --- | --- | 191.0 | 334.7 | C3'endo | 0.06 |
| 1 | 14 | U | 1061 | 43.5 | 306.8 | --- | --- | 57.9 | 165.9 | C3'endo | 0.0 |
| 1 | 14 | A | 1088 | 46.4 | 2.2 | --- | --- | 48.5 | 20.6 | C3'endo | 0.0 |
| 1 | 14 | U | 1142 | --- | --- | --- | --- | 145.9 | 358.0 | C3'endo | 0.0 |
| 1 | 14 | G | 1173 | --- | --- | --- | --- | 298.4 | 84.7 | C3'endo | 0.0 |
| 1 | 14 | A | 1274 | --- | --- | --- | --- | 9.4 | 136.6 | C3'endo | 0.01 |
| 1 | 14 | A | 1700 | --- | --- | --- | --- | 346.3 | 301.6 | C3'endo | 0.0 |
| 1 | 14 | A | 1729 | --- | --- | --- | --- | 25.1 | 142.7 | C3'endo | 0.0 |
| 1 | 14 | G | 1816 | --- | --- | --- | --- | 289.7 | 83.5 | C3'endo | 0.0 |
| 1 | 14 | G | 2116 | --- | --- | --- | --- | 48.8 | 161.9 | C3'endo | 0.0 |
| 1 | 14 | A | 2117 | --- | --- | --- | --- | 111.5 | 167.8 | C3'endo | 0.0 |
| 1 | 14 | U | 2132 | 47.0 | 1.7 | --- | --- | 107.7 | 50.8 | C3'endo | 0.0 |
| 1 | 14 | C | 2163 | --- | --- | --- | --- | 257.6 | 343.6 | C3'endo | 0.298 |
| 1 | 14 | A | 2530 | --- | --- | --- | --- | 119.1 | 141.3 | C3'endo | 0.362 |
| 1 | 14 | U | 2688 | --- | --- | --- | --- | 277.8 | 334.9 | C3'endo | 0.562 |
| 1 | 14 | A | 2799 | 201.2 | 290.5 | --- | --- | 182.6 | 340.0 | C3'endo | 0.0 |
| 1 | 14 | G | 2872 | --- | --- | --- | --- | 76.2 | 150.4 | C3'endo | 0.0 |
| 1 | 14 | A | 2883 | --- | --- | --- | --- | 153.5 | 358.7 | C3'endo | 0.0 |
| 1 | 1J | A | 15 | 138.3 | 318.0 | --- | --- | 190.8 | 342.1 | C3'endo | 0.0 |
| 1 | 13 | U | 49 | 128.7 | 334.9 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | 13 | A | 50 | 321.0 | 144.4 | 77.1 | 81.1 | --- | --- | C2'endo | 0.0 |
| 1 | 13 | A | 60 | 302.6 | 172.1 | 49.6 | 133.1 | --- | --- | C2'endo | 0.048 |
| 1 | 13 | C | 163 | 267.3 | 188.1 | 277.7 | 193.0 | --- | --- | C2'endo | 0.0 |
| 1 | 13 | G | 190 | 11.7 | 24.0 | 116.0 | 325.7 | --- | --- | C2'endo | 0.239 |
| 1 | 13 | A | 344 | 35.1 | 6.5 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | 13 | G | 484 | 26.6 | 191.9 | --- | --- | --- | --- | C2'endo | 0.053 |
| 1 | 13 | C | 748 | 132.5 | 178.0 | --- | --- | --- | --- | C2'endo | 0.437 |
| 1 | 13 | A | 815 | 67.6 | 22.9 | 53.7 | 2.0 | --- | --- | C2'endo | 0.12 |
| 1 | 13 | U | 820 | 165.5 | 2.1 | --- | --- | --- | --- | C2'endo | 0.1 |
| 1 | 13 | C | 934 | 9.9 | 68.4 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | 13 | A | 974 | 72.6 | 67.8 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | 13 | A | 975 | 37.0 | 63.0 | 40.0 | 65.5 | 66.3 | 71.9 | C2'endo | 0.0 |
| 1 | 13 | U | 1025 | 232.7 | 15.8 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | 13 | G | 1094 | 13.3 | 57.6 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | 13 | A | 1363 | 336.4 | 174.4 | 62.0 | 69.1 | --- | --- | C2'endo | 0.0 |
| 1 | 13 | C | 1397 | 330.2 | 52.5 | --- | --- | --- | --- | C2'endo | 0.157 |
| 1 | 13 | C | 1452 | 118.6 | 94.0 | 155.7 | 116.2 | --- | --- | C2'endo | 0.0 |
| 1 | 13 | A | 1502 | 148.1 | 288.8 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | 1K | A | 69 | 146.0 | 277.4 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | 2K | C | 17 | 123.2 | 4.7 | 175.1 | 313.2 | --- | --- | C2'endo | 0.0 |
| 1 | 2K | G | 19 | 258.1 | 234.1 | 326.9 | 229.3 | --- | --- | C2'endo | 0.0 |
| 1 | 4K | A | 12 | 275.2 | 230.4 | 268.3 | 221.1 | --- | --- | C2'endo | 0.0 |
| 1 | 1H | A | 196 | 303.8 | 223.0 | 325.7 | 237.6 | --- | --- | C2'endo | 0.0 |
| 1 | 1H | A | 222 | 259.4 | 257.6 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | 1H | G | 386 | 109.1 | 328.8 | 117.7 | 315.0 | --- | --- | C2'endo | 0.0 |
| 1 | 1H | C | 455 | 128.5 | 324.2 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | 1H | A | 457 | 323.0 | 187.4 | 328.9 | 162.5 | --- | --- | C2'endo | 0.0 |
| 1 | 1H | G | 481 | 157.3 | 35.2 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | 1H | A | 529 | 112.8 | 80.7 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | 1H | A | 532 | 150.5 | 26.5 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | 1H | G | 573 | 249.1 | 185.9 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | 1H | C | 574 | 45.0 | 68.1 | 70.8 | 47.9 | --- | --- | C2'endo | 0.0 |
| 1 | 1H | A | 627 | 308.6 | 193.0 | 51.5 | 159.9 | --- | --- | C2'endo | 0.0 |
| 1 | 1H | G | 974 | 76.5 | 17.2 | 116.8 | 349.0 | --- | --- | C2'endo | 0.0 |
| 1 | 1H | C | 974A | 74.4 | 8.8 | 75.9 | 11.7 | --- | --- | C2'endo | 0.0 |
| 1 | 1H | G | 1025 | 47.4 | 51.7 | 106.1 | 348.2 | --- | --- | C2'endo | 0.0 |
| 1 | 1H | U | 1033 | 9.7 | 231.5 | 15.7 | 198.2 | 349.6 | 138.7 | C2'endo | 0.0 |
| 1 | 1H | A | 1045 | 212.1 | 17.7 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | 1H | C | 1109 | 143.6 | 284.1 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | 1H | C | 1178 | 132.7 | 181.5 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | 1H | G | 1252 | 351.4 | 238.4 | --- | --- | --- | --- | C2'endo | 0.022 |
| 1 | 1H | A | 1453 | 156.4 | 333.9 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | 1H | U | 1454 | 38.8 | 211.6 | --- | --- | --- | --- | C2'endo | 0.126 |
| 1 | 1H | C | 1493 | 272.6 | 256.4 | 295.6 | 253.7 | 343.6 | 289.4 | C2'endo | 0.0 |
| 1 | 1H | A | 1609 | 2.1 | 211.6 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | 1H | A | 1729 | 54.7 | 222.0 | 79.6 | 186.7 | 26.6 | 126.2 | C2'endo | 0.0 |
| 1 | 1H | G | 1758 | 242.5 | 276.5 | 280.2 | 275.9 | --- | --- | C2'endo | 0.0 |
| 1 | 1H | A | 1913 | 55.2 | 81.4 | 70.8 | 74.6 | --- | --- | C2'endo | 0.0 |
| 1 | 1H | G | 1930 | 143.4 | 67.3 | --- | --- | --- | --- | C2'endo | 0.015 |
| 1 | 1H | A | 2031 | 115.1 | 74.7 | 127.4 | 2.3 | --- | --- | C2'endo | 0.0 |
| 1 | 1H | G | 2210 | 70.9 | 251.6 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | 1H | A | 2266 | 54.8 | 81.1 | --- | --- | --- | --- | C2'endo | 0.662 |
| 1 | 1H | G | 2307 | 85.0 | 91.3 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | 1H | A | 2346 | 340.5 | 42.4 | 354.5 | 344.4 | --- | --- | C2'endo | 0.0 |
| 1 | 1H | A | 2425 | 112.5 | 64.0 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | 1H | U | 2585 | 91.7 | 140.7 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | 1H | A | 2602 | 69.7 | 37.3 | 31.0 | 37.4 | --- | --- | C2'endo | 0.0 |
| 1 | 1H | U | 2689 | 110.3 | 55.6 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | 1H | A | 2713 | 57.5 | 243.1 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | 1H | G | 2732 | 176.3 | 12.8 | 207.1 | 344.5 | --- | --- | C2'endo | 0.01 |
| 1 | 1H | U | 2756 | 133.7 | 144.4 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | 1H | A | 2790 | 231.9 | 345.3 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | 1G | A | 51 | 16.7 | 65.4 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | 1G | A | 60 | 300.8 | 174.1 | 50.1 | 131.3 | --- | --- | C2'endo | 0.316 |
| 1 | 1G | G | 190 | 359.5 | 29.4 | 105.5 | 340.8 | --- | --- | C2'endo | 0.0 |
| 1 | 1G | U | 209 | 273.3 | 202.6 | 292.4 | 205.4 | --- | --- | C2'endo | 0.0 |
| 1 | 1G | A | 412 | 14.3 | 26.8 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | 1G | U | 421 | 86.3 | 352.8 | --- | --- | --- | --- | C2'endo | 0.047 |
| 1 | 1G | A | 532 | 293.4 | 292.8 | 287.7 | 223.3 | --- | --- | C2'endo | 0.0 |
| 1 | 1G | A | 815 | 74.2 | 16.9 | 59.0 | 358.9 | --- | --- | C2'endo | 0.206 |
| 1 | 1G | U | 820 | 153.4 | 24.5 | 209.6 | 358.0 | --- | --- | C2'endo | 0.385 |
| 1 | 1G | A | 974 | 88.5 | 66.5 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | 1G | U | 982 | 91.3 | 90.8 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | 1G | U | 1126 | 343.4 | 255.2 | 237.1 | 352.1 | --- | --- | C2'endo | 0.0 |
| 1 | 1G | G | 1139 | 4.6 | 58.4 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | 1G | A | 1157 | 125.1 | 30.6 | 91.6 | 67.8 | 70.2 | 72.2 | C2'endo | 0.0 |
| 1 | 1G | A | 1201 | 37.6 | 101.2 | 34.1 | 96.6 | --- | --- | C2'endo | 0.0 |
| 1 | 1G | A | 1502 | 143.1 | 287.5 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | 1G | G | 1529 | 88.9 | 358.5 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | 1L | U | 7 | 167.5 | 355.4 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | 1L | A | 9 | 258.6 | 288.4 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | 2L | C | 17 | 135.4 | 329.0 | 186.7 | 296.2 | --- | --- | C2'endo | 0.012 |
| 1 | 3L | U | 33 | 131.9 | 150.2 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | 4L | A | 12 | 321.9 | 247.0 | 321.1 | 256.8 | 305.7 | 250.6 | C2'endo | 0.0 |
| 1 | 4L | A | 14 | 254.0 | 220.9 | 268.6 | 229.1 | --- | --- | C2'endo | 0.0 |
| 1 | 14 | A | 196 | 305.4 | 224.5 | 328.8 | 238.7 | --- | --- | C2'endo | 0.0 |
| 1 | 14 | A | 322 | 164.3 | 341.1 | --- | --- | --- | --- | C2'endo | 0.066 |
| 1 | 14 | A | 330 | 81.9 | 242.9 | 137.8 | 201.0 | --- | --- | C2'endo | 0.0 |
| 1 | 14 | G | 386 | 114.2 | 336.4 | 120.2 | 324.1 | --- | --- | C2'endo | 0.0 |
| 1 | 14 | G | 481 | 156.7 | 33.4 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | 14 | A | 529 | 121.5 | 81.4 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | 14 | A | 532 | 151.2 | 34.2 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | 14 | U | 562 | 303.6 | 156.2 | 314.6 | 141.2 | --- | --- | C2'endo | 0.016 |
| 1 | 14 | G | 573 | 246.8 | 189.6 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | 14 | C | 574 | 46.6 | 71.2 | 71.0 | 51.8 | --- | --- | C2'endo | 0.0 |
| 1 | 14 | A | 616 | 336.1 | 228.0 | --- | --- | --- | --- | C2'endo | 0.094 |
| 1 | 14 | A | 627 | 293.7 | 202.9 | 51.0 | 163.0 | --- | --- | C2'endo | 0.0 |
| 1 | 14 | A | 945 | 92.2 | 224.3 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | 14 | C | 974A | 62.5 | 23.1 | 59.8 | 18.5 | --- | --- | C2'endo | 0.137 |
| 1 | 14 | U | 1012 | 282.5 | 29.3 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | 14 | G | 1025 | 51.7 | 54.1 | 106.4 | 325.8 | --- | --- | C2'endo | 0.0 |
| 1 | 14 | A | 1609 | 1.1 | 210.2 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | 14 | G | 1758 | 251.6 | 270.1 | 287.2 | 274.1 | --- | --- | C2'endo | 0.131 |
| 1 | 14 | A | 1913 | 91.4 | 72.5 | 85.0 | 82.5 | --- | --- | C2'endo | 0.0 |
| 1 | 14 | G | 1930 | 144.3 | 71.0 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | 14 | A | 1938 | 14.4 | 254.8 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | 14 | U | 1963 | 78.6 | 223.8 | 46.9 | 192.2 | --- | --- | C2'endo | 0.0 |
| 1 | 14 | G | 1964 | 290.2 | 353.6 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | 14 | A | 2031 | 106.4 | 78.8 | 121.9 | 13.9 | --- | --- | C2'endo | 0.0 |
| 1 | 14 | G | 2032 | 298.7 | 231.3 | 6.3 | 192.0 | --- | --- | C2'endo | 0.022 |
| 1 | 14 | A | 2033 | 327.8 | 238.2 | 355.5 | 176.4 | --- | --- | C2'endo | 0.0 |
| 1 | 14 | G | 2211 | 128.5 | 358.7 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | 14 | A | 2212 | 119.8 | 95.9 | 170.6 | 62.5 | --- | --- | C2'endo | 0.0 |
| 1 | 14 | A | 2266 | 62.3 | 71.9 | --- | --- | --- | --- | C2'endo | 0.413 |
| 1 | 14 | A | 2346 | 353.5 | 38.7 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | 14 | A | 2425 | 111.2 | 57.2 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | 14 | U | 2585 | 94.5 | 144.3 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | 14 | A | 2602 | 70.7 | 26.1 | 40.7 | 24.1 | 0.2 | 28.2 | C2'endo | 0.0 |
| 1 | 14 | A | 2713 | 55.0 | 242.4 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | 14 | G | 2732 | 173.7 | 12.2 | 203.4 | 345.4 | --- | --- | C2'endo | 0.0 |
| 1 | 14 | G | 2751 | 37.3 | 248.5 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | 14 | U | 2756 | 105.3 | 163.1 | --- | --- | --- | --- | C2'endo | 0.543 |
| 1 | 14 | A | 2790 | 280.4 | 258.3 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | 1J | C | 88 | 26.7 | 234.7 | 314.1 | 305.8 | 333.5 | 351.9 | C2'endo | 0.0 |
| 1 | 1J | C | 108 | 223.8 | 30.7 | --- | --- | --- | --- | C2'endo | 0.0 |
| 1 | 13 | A | 8 | --- | --- | 294.0 | 243.3 | 307.6 | 263.4 | C2'endo | 0.0 |
| 1 | 13 | U | 189 | --- | --- | 318.0 | 251.4 | --- | --- | C2'endo | 0.0 |
| 1 | 13 | A | 451 | --- | --- | 78.9 | 98.1 | --- | --- | C2'endo | 0.132 |
| 1 | 13 | A | 532 | --- | --- | 296.7 | 239.5 | 289.5 | 259.3 | C2'endo | 0.0 |
| 1 | 13 | U | 1196 | --- | --- | 297.7 | 34.0 | 323.7 | 115.9 | C2'endo | 0.0 |
| 1 | 13 | A | 1201 | --- | --- | 45.1 | 89.8 | --- | --- | C2'endo | 0.0 |
| 1 | 1H | A | 265 | --- | --- | 81.4 | 160.0 | --- | --- | C2'endo | 0.307 |
| 1 | 1H | A | 330 | --- | --- | 143.8 | 204.3 | --- | --- | C2'endo | 0.0 |
| 1 | 1H | C | 456 | --- | --- | 23.1 | 150.2 | --- | --- | C2'endo | 0.0 |
| 1 | 1H | G | 530 | --- | --- | 171.1 | 199.4 | --- | --- | C2'endo | 0.0 |
| 1 | 1H | U | 614 | --- | --- | 67.9 | 239.7 | --- | --- | C2'endo | 0.0 |
| 1 | 1H | A | 957 | --- | --- | 85.4 | 209.4 | --- | --- | C2'endo | 0.262 |
| 1 | 1H | G | 1325 | --- | --- | 335.5 | 244.2 | --- | --- | C2'endo | 0.1 |
| 1 | 1H | G | 1416 | --- | --- | 320.9 | 137.7 | --- | --- | C2'endo | 0.0 |
| 1 | 1H | A | 2033 | --- | --- | 335.6 | 182.4 | --- | --- | C2'endo | 0.0 |
| 1 | 1H | G | 2345 | --- | --- | 38.1 | 67.6 | 32.3 | 79.4 | C2'endo | 0.0 |
| 1 | 1H | U | 2702 | --- | --- | 85.8 | 145.9 | 47.9 | 138.9 | C2'endo | 0.0 |
| 1 | 1H | U | 2712 | --- | --- | 24.6 | 164.1 | 101.2 | 108.5 | C2'endo | 0.683 |
| 1 | 1G | A | 8 | --- | --- | 297.1 | 245.1 | 316.8 | 259.7 | C2'endo | 0.395 |
| 1 | 1G | A | 50 | --- | --- | 77.5 | 84.9 | --- | --- | C2'endo | 0.0 |
| 1 | 1G | U | 189 | --- | --- | 333.3 | 251.4 | 311.1 | 217.6 | C2'endo | 0.0 |
| 1 | 1G | A | 451 | --- | --- | 84.7 | 100.7 | --- | --- | C2'endo | 0.191 |
| 1 | 1G | A | 496 | --- | --- | 161.4 | 210.9 | --- | --- | C2'endo | 0.0 |
| 1 | 1G | U | 531 | --- | --- | 315.5 | 150.7 | --- | --- | C2'endo | 0.0 |
| 1 | 1G | A | 1363 | --- | --- | 83.0 | 53.5 | --- | --- | C2'endo | 0.01 |
| 1 | 1G | C | 1533 | --- | --- | 280.5 | 329.1 | 287.4 | 35.6 | C2'endo | 0.0 |
| 1 | 2L | G | 19 | --- | --- | 324.9 | 233.7 | 342.0 | 259.7 | C2'endo | 0.016 |
| 1 | 14 | A | 265 | --- | --- | 81.7 | 153.3 | --- | --- | C2'endo | 0.0 |
| 1 | 14 | A | 457 | --- | --- | 16.0 | 146.5 | --- | --- | C2'endo | 0.0 |
| 1 | 14 | G | 530 | --- | --- | 171.6 | 198.9 | --- | --- | C2'endo | 0.0 |
| 1 | 14 | G | 1252 | --- | --- | 41.5 | 199.7 | --- | --- | C2'endo | 0.055 |
| 1 | 14 | G | 1325 | --- | --- | 337.4 | 246.3 | --- | --- | C2'endo | 0.0 |
| 1 | 14 | C | 1493 | --- | --- | 289.1 | 267.3 | 338.9 | 296.7 | C2'endo | 0.0 |
| 1 | 14 | G | 1534 | --- | --- | 271.6 | 265.1 | 328.6 | 205.9 | C2'endo | 0.0 |
| 1 | 14 | U | 2712 | --- | --- | 25.7 | 163.3 | 101.1 | 107.4 | C2'endo | 0.274 |
| 1 | 13 | A | 51 | 44.0 | 50.6 | --- | --- | 348.9 | 29.8 | C2'endo | 0.0 |
| 1 | 13 | U | 65 | 19.0 | 85.8 | --- | --- | 14.0 | 8.4 | C2'endo | 0.0 |
| 1 | 13 | G | 129A | --- | --- | --- | --- | 295.1 | 24.8 | C2'endo | 0.0 |
| 1 | 13 | G | 388 | --- | --- | --- | --- | 280.3 | 36.1 | C2'endo | 0.0 |
| 1 | 13 | A | 533 | 274.0 | 284.9 | --- | --- | 332.5 | 193.8 | C2'endo | 0.0 |
| 1 | 13 | U | 561 | --- | --- | --- | --- | 66.0 | 114.8 | C2'endo | 0.0 |
| 1 | 13 | C | 1054 | --- | --- | --- | --- | 349.1 | 107.5 | C2'endo | 0.16 |
| 1 | 3K | U | 34 | 6.4 | 203.7 | --- | --- | 337.9 | 261.7 | C2'endo | 0.0 |
| 1 | 1H | G | 321 | --- | --- | --- | --- | 338.7 | 298.7 | C2'endo | 0.088 |
| 1 | 1H | G | 323 | 98.3 | 64.6 | --- | --- | 159.2 | 29.6 | C2'endo | 0.0 |
| 1 | 1H | C | 995 | --- | --- | --- | --- | 350.0 | 74.4 | C2'endo | 0.055 |
| 1 | 1H | U | 1205 | --- | --- | --- | --- | 0.6 | 9.6 | C2'endo | 0.0 |
| 1 | 1H | C | 1428 | --- | --- | --- | --- | 151.1 | 286.9 | C2'endo | 0.0 |
| 1 | 1H | A | 1460 | --- | --- | --- | --- | 338.2 | 87.6 | C2'endo | 0.0 |
| 1 | 1H | A | 1543 | --- | --- | --- | --- | 101.6 | 76.9 | C2'endo | 0.0 |
| 1 | 1H | G | 1674 | --- | --- | --- | --- | 334.1 | 96.1 | C2'endo | 0.0 |
| 1 | 1H | A | 1780 | --- | --- | --- | --- | 278.0 | 275.2 | C2'endo | 0.223 |
| 1 | 1H | A | 1847 | 277.2 | 346.2 | --- | --- | 305.3 | 346.0 | C2'endo | 0.0 |
| 1 | 1H | A | 1900 | --- | --- | --- | --- | 302.9 | 17.6 | C2'endo | 0.0 |
| 1 | 1H | U | 2249 | --- | --- | --- | --- | 300.3 | 259.8 | C2'endo | 0.339 |
| 1 | 1H | G | 2384 | --- | --- | --- | --- | 260.6 | 263.5 | C2'endo | 0.768 |
| 1 | 1G | G | 129A | --- | --- | --- | --- | 258.8 | 20.9 | C2'endo | 0.01 |
| 1 | 1G | A | 533 | 283.0 | 287.4 | --- | --- | 353.2 | 187.2 | C2'endo | 0.0 |
| 1 | 1G | U | 561 | --- | --- | --- | --- | 60.9 | 117.1 | C2'endo | 0.0 |
| 1 | 1G | A | 975 | 18.8 | 78.2 | --- | --- | 74.0 | 71.4 | C2'endo | 0.0 |
| 1 | 1G | U | 1049 | --- | --- | --- | --- | 349.1 | 32.2 | C2'endo | 0.24 |
| 1 | 1G | C | 1054 | --- | --- | --- | --- | 357.7 | 124.0 | C2'endo | 0.0 |
| 1 | 1G | G | 1064 | --- | --- | --- | --- | 96.0 | 129.9 | C2'endo | 0.021 |
| 1 | 1G | A | 1101 | --- | --- | --- | --- | 331.9 | 142.8 | C2'endo | 0.0 |
| 1 | 1G | G | 1177 | --- | --- | --- | --- | 263.3 | 275.9 | C2'endo | 0.0 |
| 1 | 1G | U | 1240 | --- | --- | --- | --- | 2.1 | 8.7 | C2'endo | 0.0 |
| 1 | 3L | U | 7 | --- | --- | --- | --- | 271.4 | 282.6 | C2'endo | 0.16 |
| 1 | 3L | A | 9 | --- | --- | --- | --- | 148.7 | 274.8 | C2'endo | 0.659 |
| 1 | 14 | A | 278 | 147.1 | 287.5 | --- | --- | 261.7 | 263.0 | C2'endo | 0.0 |
| 1 | 14 | G | 321 | --- | --- | --- | --- | 332.8 | 299.9 | C2'endo | 0.168 |
| 1 | 14 | G | 323 | 93.6 | 54.4 | --- | --- | 156.9 | 27.4 | C2'endo | 0.152 |
| 1 | 14 | U | 1205 | --- | --- | --- | --- | 358.3 | 17.9 | C2'endo | 0.0 |
| 1 | 14 | A | 1460 | --- | --- | --- | --- | 9.1 | 38.0 | C2'endo | 0.0 |
| 1 | 14 | G | 1674 | --- | --- | --- | --- | 334.2 | 96.8 | C2'endo | 0.0 |
| 1 | 14 | A | 1780 | --- | --- | --- | --- | 272.4 | 253.7 | C2'endo | 0.0 |
| 1 | 14 | A | 1847 | --- | --- | --- | --- | 322.4 | 339.1 | C2'endo | 0.0 |
| 1 | 14 | A | 1900 | --- | --- | --- | --- | 316.7 | 16.8 | C2'endo | 0.0 |
| 1 | 14 | U | 2092 | --- | --- | --- | --- | 75.0 | 118.8 | C2'endo | 0.477 |
| 1 | 14 | U | 2249 | --- | --- | --- | --- | 300.0 | 249.3 | C2'endo | 0.0 |
| 1 | 14 | G | 2345 | 33.3 | 104.2 | --- | --- | 26.4 | 79.6 | C2'endo | 0.131 |
| 1 | 14 | U | 2702 | --- | --- | --- | --- | 54.3 | 132.7 | C2'endo | 0.0 |